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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 11

sp. nov., isolated from deep seawater of the Mariana Trench Free

Abstract

A Gram-stain-negative, aerobic, polarly flagellated, straight or curved rod-shaped bacterium, designated strain M1K-6, was isolated from deep seawater samples collected from the Mariana Trench. The strain grew at −4 to 37 °C (optimum, 25–30 °C), at pH 5.5–10.0 (optimum, pH 7.0) and with 0.5–14.0  % (w/v) NaCl (optimum, 2.0 %). It did not reduce nitrate to nitrite nor hydrolyse gelatin or starch. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M1K-6 was affiliated with the genus , sharing 93.1–97.0  % sequence similarity with the type strains of recognized species. The major cellular fatty acids were summed feature 3 (C 6/C 7), summed feature 8 (C 7/C 6), C, C 3-OH and C. The predominant respiratory quinone was ubiquinone-8. Polar lipids of strain M1K-6 included phosphatidylethanolamine, phosphatidylglycerol and two unidentified lipids. The genomic G+C content of strain M1K-6 was 46.0 mol%. Based on data from the present polyphasic study, strain M1K-6 was considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is M1K-6 (=KCTC 72501=MCCC 1K03890).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): deep seawater , Mariana Trench and Marinomonas profundi sp. nov.
Funding
This study was supported by the:
  • the Science and Technology Basic Resources Investigation Program of China (Award 2017FY100804)
    • Principle Award Recipient: xi-ying zhang
  • Taishan Scholars Program of Shandong Province (Award tspd20181203)
    • Principle Award Recipient: Yu-Zhong Zhang
  • National Science Foundation of China (Award 31870052)
    • Principle Award Recipient: Xiu-Lan Chen
  • AoShan Talents Cultivation Program (Award 2017ASTCP-OS14)
    • Principle Award Recipient: Yu-Zhong Zhang
  • National Key R&D Program of China (Award 2018YFC1406704)
    • Principle Award Recipient: xi-ying zhang
  • National Key R&D Program of China (Award 2018YFC1406703)
    • Principle Award Recipient: Yu-Zhong Zhang
  • National Key R&D Program of China (Award 2018YFC1406701)
    • Principle Award Recipient: Hai-Nan Su
  • National Key R&D Program of China (Award 2018YFC1406504)
    • Principle Award Recipient: Xiao-Yan Song
  • National Key R&D Program of China (Award 2018YFC0310704)
    • Principle Award Recipient: Xiu-Lan Chen
© 2020 The Authors
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Marinomonas profundi sp. nov., isolated from deep seawater of the Mariana Trench
Int J Syst Evol Microbiol 70, 5747 (2020); https://doi.org/10.1099/ijsem.0.004472
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