1887

Abstract

A Gram-stain-negative, aerobic, polarly flagellated, straight or curved rod-shaped bacterium, designated strain M1K-6, was isolated from deep seawater samples collected from the Mariana Trench. The strain grew at −4 to 37 °C (optimum, 25–30 °C), at pH 5.5–10.0 (optimum, pH 7.0) and with 0.5–14.0  % (w/v) NaCl (optimum, 2.0 %). It did not reduce nitrate to nitrite nor hydrolyse gelatin or starch. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain M1K-6 was affiliated with the genus , sharing 93.1–97.0  % sequence similarity with the type strains of recognized species. The major cellular fatty acids were summed feature 3 (C 6/C 7), summed feature 8 (C 7/C 6), C, C 3-OH and C. The predominant respiratory quinone was ubiquinone-8. Polar lipids of strain M1K-6 included phosphatidylethanolamine, phosphatidylglycerol and two unidentified lipids. The genomic G+C content of strain M1K-6 was 46.0 mol%. Based on data from the present polyphasic study, strain M1K-6 was considered to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is M1K-6 (=KCTC 72501=MCCC 1K03890).

Funding
This study was supported by the:
  • xi-ying zhang , the Science and Technology Basic Resources Investigation Program of China , (Award 2017FY100804)
  • Yu-Zhong Zhang , Taishan Scholars Program of Shandong Province , (Award tspd20181203)
  • Xiu-Lan Chen , National Science Foundation of China , (Award 31870052)
  • Yu-Zhong Zhang , AoShan Talents Cultivation Program , (Award 2017ASTCP-OS14)
  • xi-ying zhang , National Key R&D Program of China , (Award 2018YFC1406704)
  • Yu-Zhong Zhang , National Key R&D Program of China , (Award 2018YFC1406703)
  • Hai-Nan Su , National Key R&D Program of China , (Award 2018YFC1406701)
  • Xiao-Yan Song , National Key R&D Program of China , (Award 2018YFC1406504)
  • Xiu-Lan Chen , National Key R&D Program of China , (Award 2018YFC0310704)
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2020-09-18
2021-03-02
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