1887

Abstract

The taxonomic classification of species has been revised and updated several times. This study utilized average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) cutoff values of 95 and 70 %, respectively, to re-identify the species of strains deposited in GenBank as , and . Of the 264 deposited strains, 259 were correctly identified as , but the remaining five were not. All 28 deposited strains had been incorrectly identified as . Four of these strains were re-identified, including two as and one each as and , but the remaining 24 could not be re-identified. Similarly, all 35 deposited strains had been incorrectly identified as . Nineteen of these strains were re-identified, including 12 as , four as and one each as , and . These results strongly suggest that bacteria should be identified using ANI and dDDH analyses based on whole genome sequencing when species are initially deposited in GenBank/DDBJ/EMBL databases.

Funding
This study was supported by the:
  • Teruo Kirikae , Japan Agency for Medical Research and Development , (Award 19fk0108061h0302)
  • Teruo Kirikae , Japan Society for the Promotion of Science , (Award 19KK0203)
  • Mari Tohya , Japan Society for the Promotion of Science , (Award 19K16652)
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/content/journal/ijsem/10.1099/ijsem.0.004468
2020-09-16
2020-09-22
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