%0 Journal Article %A Yin, Xue-Meng %A Yang, Xiao-Yan %A Hou, Jing %A Zhu, Lin %A Cui, Heng-Lin %T Natronomonas halophila sp. nov. and Natronomonas salina sp. nov., two novel halophilic archaea %D 2020 %J International Journal of Systematic and Evolutionary Microbiology, %V 70 %N 11 %P 5686-5692 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.004463 %K salt mine %K halophilic archaea %K salt lake %K Natronomonas salina sp. nov. %K Natronomonas halophila sp. nov. %I Microbiology Society, %X Two halophilic archaeal strains, C90T and YPL13T, were isolated from a salt lake and a salt mine in PR China. The two strains were found to form two clusters (97.5 and 89.5 % similarity between them, respectively) separating them from the three current members of the genus Natronomonas (95.4–97.0 % and 86.6–89.3 % similarity, respectively) on the basis of the 16S rRNA and rpoB′ gene sequence similarities and phylogenetic analysis. Diverse phenotypic characteristics differentiate strains C90T and YPL13T from current Natronomonas members. The polar lipids of strain C90T were phosphatidic acid, phosphatidylglycerol (PG), phosphatidylglycerol phosphate methyl ester (PGP-Me), phosphatidylglycerol sulphate, two unidentified glycolipids, a major glycolipid and a minor glycolipid, while those of strain YPL13T were PG, PGP-Me, two unidentified phospholipids and a glycolipid. The average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH) values between the two strains were 79.8 and 27.1 %, respectively, which were much lower than the threshold values proposed as a species boundaries (ANI 95–96 % and isDDH 70 %), which revealed that the two strains represent two novel species; these values (ANI 76.6–80.0 % and isDDH 21.6–27.0 %) of the strains examined in this study and the current members of Natronomonas are much lower than the recommended threshold values, suggesting that strains C90T and YPL13T represent two genomically different species of Natronomonas . These results showed that strains C90T (=CGMCC 1.13738T=JCM 32961T) and YPL13T (=CGMCC 1.13884T=JCM 31111T) represent two novel species of Natronomonas , for which the names Natronomonas halophila sp. nov. and Natronomonas salina sp. nov. are proposed. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004463