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Volume 70, Issue 11

sp. nov., isolated from a Yellow River sediment sample Free

Abstract

Strain CFH S0501, a novel Gram-stain-positive, aerobic, rod-shaped, endospore-forming and motile micro-organism with peritrichous flagella, was isolated from a sediment sample collected from the Yellow River in Henan Province, PR China. Optimum growth was observed at 28 °C, pH 7.0 and without NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences indicated that the strain belonged to the genus and was closely related to DSM 8445 and Gsoil 3088 (with 96.8 and 96.7 % sequence similarity, respectively). The predominant menaquinone was MK-7. Major cellular fatty acids were anteiso-C and iso-C. Polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmonomethylethanolamine, phosphatidylethanolamine, two unidentified phospholipids and an unidentified polar lipid. The cell-wall peptidoglycan was found to contain meso-diaminopimelic acid. The genome size was 5.26 Mbp with a G+C content of 49.7 mol%. The average nucleotide identity (ANI) and DNA–DNAhybridization (DDH) values between CFH S0501 and the other species of the genus were found to be low (ANIm <86.11 %, ANIb <70.30 % and DDH <25.00 %). Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH S0501 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is CFH S0501 (=DSM 29940=BCRC 80809).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Brevibacillus migulae sp. nov. , polyphasic taxonomy and Yellow River
Funding
This study was supported by the:
  • Doctor Scientific Research Fund of Xinxiang Medical University (Award XYBSKYZZ201625)
    • Principle Award Recipient: Hong Ming
  • Henan Province University youth researcher support project (Award 2017GGJS106)
    • Principle Award Recipient: Hong Ming
  • Innovation Scientists and Technicians Troop Construction Projects of Henan Province (Award CXTD2016043)
    • Principle Award Recipient: Guo-Xing Nie
  • Key Technologies R&D Program of Henan Province (Award 202102110107)
    • Principle Award Recipient: Hong Ming
© 2020 The Authors
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2020-09-15
2024-04-19
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Brevibacillus migulae sp. nov., isolated from a Yellow River sediment sample
Int J Syst Evol Microbiol 70, 5693 (2020); https://doi.org/10.1099/ijsem.0.004462
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