1887

Abstract

Nine Gram-stain-positive cocci, coccobacilli or short, rod-shaped strains recovered from clinical sources from patients located in two Canadian provinces and one environmental source were extensively studied. Clinical sources included blood cultures, cerebral spinal fluid, lymph node, lung biopsy and peritoneal fluid. Through 16S rRNA gene and whole genome sequencing analyses, the strains were found to cluster into three groups, closest to but distinguished from other genera in the family . The genomes from these bacteria had high G+C content, ranging from 67.8–69.56 mol%, and genome sizes of 3.02–4.52 Mb. Biochemical and chemotaxonomic properties including branched-chain cellular fatty acids, -lysine diaminopimelic acid (-DAP) and cell-wall type A3γ (-DAP-gly) containing -DAP, alanine, glycine and glutamic acid were found and so the strains were therefore deemed to be consistent with other new genera in this family. Based on this investigation, we propose gen. nov., sp. nov., sp. nov. and gen. nov., sp. nov. for these taxa. Misidentified taxon ‘’ was found to be assignable to based on this study.

Funding
This study was supported by the:
  • Kathryn A. Bernard , Public Health Agency of Canada-A Base
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004461
2020-09-15
2020-09-20
Loading full text...

Full text loading...

References

  1. Sun Y, Wang H, Zhang T, Liu W-H, Liu H-Y et al. Desertihabitans aurantiacus gen. nov., sp. nov., a novel member of the family Propionibacteriaceae. Int J Syst Evol Microbiol 2019; 69:2486–2491 [CrossRef][PubMed]
    [Google Scholar]
  2. Wenning M, Breitenwieser F, Huptas C, Doll E, Bächler B et al. Brevilactibacter flavus gen. nov., sp. nov., a novel bacterium of the family Propionibacteriaceae isolated from raw milk and dairy products and reclassification of Propioniciclava sinopodophylli as Brevilactibacter sinopodophylli comb. nov. Int J Syst Evol Microbiol 2020; 70:2186–2193 [CrossRef][PubMed]
    [Google Scholar]
  3. Yokota A et al. Genus XI. Propioniferax Yokata, Tamura, Takeuchi, Weiss and Stackebrandt 1994, 581VP. In Whitman WB, Goodfellow M, Kämpfer P. (editors) Bergeys Manual of Systematic Bacteriology. Volume 5: The Actinobacteria New York: Springer.; 2012 pp 1177–1179
    [Google Scholar]
  4. Bernard KA, Shuttleworth L, Munro C, Forbes-Faulkner JC, Pitt D et al. Propionibacterium australiense sp. nov. derived from granulomatous bovine lesions. Anaerobe 2002; 8:41–47 [CrossRef]
    [Google Scholar]
  5. Stackebrandt E, Ebers J. Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 2006; 33:152–155
    [Google Scholar]
  6. Lee DW, Lee SD. Ponticoccus gilvus gen. nov., sp. nov., a novel member of the family Propionibacteriaceae from seawater. J Microbiol 2008; 46:508–512 [CrossRef][PubMed]
    [Google Scholar]
  7. Hwang CY, Cho BC. Ponticoccus litoralis gen. nov., sp. nov., a marine bacterium in the family Rhodobacteraceae. Int J Syst Evol Microbiol 2008; 58:1332–1338 [CrossRef][PubMed]
    [Google Scholar]
  8. Bernier A-M, Bernard K. Draft genome sequence of Trueperella bernardiae LCDC 89-0504T, isolated from a human blood culture. Genome Announc 2016; 4:e01634-15 [CrossRef][PubMed]
    [Google Scholar]
  9. Seemann T. Prokka: rapid prokaryotic genome annotation. Bioinformatics 2014; 30:2068–2069 [CrossRef][PubMed]
    [Google Scholar]
  10. Meier-Kolthoff JP, Klenk H-P, Göker M. Taxonomic use of DNA G+C content and DNA-DNA hybridization in the genomic age. Int J Syst Evol Microbiol 2014; 64:352–356 [CrossRef][PubMed]
    [Google Scholar]
  11. Rodriguez-R LM, Konstantinidis KT. Bypassing cultivation to identify bacterial species. Microbe 2014; 9:111–118 [CrossRef]
    [Google Scholar]
  12. Nicholson AC, Gulvik CA, Whitney AM, Humrighouse BW, Bell ME et al. Division of the genus Chryseobacterium: Observation of discontinuities in amino acid identity values, a possible consequence of major extinction events, guides transfer of nine species to the genus Epilithonimonas, eleven species to the genus Kaistella, and three species to the genus Halpernia gen. nov., with description of Kaistella daneshvariae sp. nov. and Epilithonimonas vandammei sp. nov. derived from clinical specimens. Int J Syst Evol Microbiol 2020; 70:4432–4450 [CrossRef][PubMed]
    [Google Scholar]
  13. Verroken A, Janssens M, Berhin C, Bogaerts P, Huang T-D et al. Evaluation of matrix-assisted laser desorption ionization-time of flight mass spectrometry for identification of Nocardia species. J Clin Microbiol 2010; 48:4015–4021 [CrossRef][PubMed]
    [Google Scholar]
  14. Bernard KA, Pacheco AL, Loomer C, Burdz T, Wiebe D et al. Corynebacterium lowii sp. nov. and Corynebacterium oculi sp. nov., derived from human clinical disease and an emended description of Corynebacterium mastitidis. Int J Syst Evol Microbiol 2016; 66:2803–2812 [CrossRef][PubMed]
    [Google Scholar]
  15. Weyant RS, Moss CW, Weaver RE, Hollis DG, Jordan JG et al. Identification of Unusual Pathogenic Gram-Negative Aerobic and Facultatively Anaerobic Bacteria, 2nd ed. Baltimore, MD: Williams & Wilkins; 1996
    [Google Scholar]
  16. Bernard KA, Munro C, Wiebe D, Ongsansoy E. Characteristics of rare or recently described Corynebacterium species recovered from human clinical material in Canada. J Clin Microbiol 2002; 40:4375–4381 [CrossRef][PubMed]
    [Google Scholar]
  17. Bernard KA, Bellefeuille M, Ewan EP. Cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods. J Clin Microbiol 1991; 29:83–89 [CrossRef][PubMed]
    [Google Scholar]
  18. Schumann P. Peptidoglycan structure in methods in microbiology vol 38. In Rainey FA, Oren A. (editors) The Taxonomy of Prokaryotes United Kingdom: Elsevier; 2011 pp 101–129
    [Google Scholar]
  19. Hasegawa T, Takizawa M, Tanida S. A rapid analysis for chemical grouping of aerobic actinomycetes. J Gen Appl Microbiol 1983; 29:319–322 [CrossRef]
    [Google Scholar]
  20. Bernard K, Pacheco AL. In vitro activity of 22 antimicrobial agents against Corynebacterium and Microbacterium species referred to the Canadian national microbiology laboratory. Clinical Microbiology Newsletter 2015; 37:187–198 [CrossRef]
    [Google Scholar]
  21. Clinical Laboratory Standards Institute M45-3. Methods for Antimicrobial Dilution and Disk Susceptibility Testing of Infrequently Isolated or Fastidious Bacteria, 3rd ed. Wayne, Pa: Clinical Laboratory Standards Institute; 2016
    [Google Scholar]
  22. Alcock BP, Raphenya AR, Lau TTY, Tsang KK, Bouchard M et al. CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database. Nucleic Acids Res 2020; 48:D517–525 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004461
Loading
/content/journal/ijsem/10.1099/ijsem.0.004461
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error