1887

Abstract

A Gram-stain-positive, aerobic, motile, coccus-shaped bacterium, designated strain SCSIO 13315, was isolated from mucus of the coral sp. collected from Luhuitou fringing reef (Sanya, Hainan, PR China). Analysis of the 16S rRNA gene sequence showed that strain SCSIO 13315 exhibits 95.5 % 16S rRNA gene sequence similarity to SKC1-2, 95.8 % to T2A-S27 and 96.4 % to KST3-3. Results of phylogenetic analysis suggested that strain SCSIO 13315 was a member of the genus . The DNA G+C content of strain SCSIO 13315 was 73.5 %. Chemotaxonomic assessment of strain SCSIO 13315 showed that the menaquinones were MK-8(H) and MK-9(H). The main cellular fatty acids were anteiso-C, C and summed feature 3 (C 7/C 6). The polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, two unknown phospholipids, four unidentified aminolipids and one unidentified lipid. Based on the phylogenetic and phenotypic analysis, it was evident that strain SCSIO 13315 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCSIO 13315 (=NBRC 109944=DSM 27812).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41676155)
    • Principle Award Recipient: Jie Li
  • National Natural Science Foundation of China (Award 41890853)
    • Principle Award Recipient: Si Zhang
  • National Key R&D Program of China (Award 2017YFC0506303)
    • Principle Award Recipient: Jie Li
  • Guangdong Natural Science Funds for Distinguished Young Scholar (Award 2017A030306025)
    • Principle Award Recipient: Jie Li
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004460
2020-09-08
2021-07-27
Loading full text...

Full text loading...

References

  1. Jurado V, Laiz L, Ortiz-Martinez A, Groth I, Saiz-Jimenez C. Pseudokineococcus lusitanus gen. nov., sp. nov., and reclassification of Kineococcus marinus Lee 2006 as Pseudokineococcus marinus comb. nov. Int J Syst Evol Microbiol 2011; 61:2515–2519 [View Article][PubMed]
    [Google Scholar]
  2. Lee SD. Kineococcus marinus sp. nov., isolated from marine sediment of the coast of Jeju, Korea. Int J Syst Evol Microbiol 2006; 56:1279–1283 [View Article][PubMed]
    [Google Scholar]
  3. Lee DW, Park MY, Kim J-J, Kim BS. Pseudokineococcus basanitobsidens sp. nov., isolated from volcanic rock. Int J Syst Evol Microbiol 2017; 67:3824–3828 [View Article][PubMed]
    [Google Scholar]
  4. Woodhead AJ, Hicks CC, Norstrom AV, Williams GJ, Graham NAJ. Coral reef ecosystem services in the Anthropocene. Funct Ecol 2019; 33:1023–1034
    [Google Scholar]
  5. Wild C, Huettel M, Klueter A, Kremb SG, Rasheed MYM et al. Coral mucus functions as an energy carrier and particle trap in the reef ecosystem. Nature 2004; 428:66–70 [View Article][PubMed]
    [Google Scholar]
  6. You Z-Q, Li J, Qin S, Tian X-P, Wang F-Z et al. Georgenia sediminis sp. nov., a moderately thermophilic actinobacterium isolated from sediment. Int J Syst Evol Microbiol 2013; 63:4243–4247 [View Article][PubMed]
    [Google Scholar]
  7. Zhang L, Xi L, Ruan J, Huang Y. Kocuria oceani sp. nov., isolated from a deep-sea hydrothermal plume. Int J Syst Evol Microbiol 2017; 67:164–169 [View Article][PubMed]
    [Google Scholar]
  8. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  10. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
    [Google Scholar]
  11. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article][PubMed]
    [Google Scholar]
  12. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  13. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  14. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  15. Lee I, Ouk Kim Y, Park S-C, Chun J. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol 2016; 66:1100–1103 [View Article][PubMed]
    [Google Scholar]
  16. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 2013; 14:60 [View Article][PubMed]
    [Google Scholar]
  17. Li J, Zhang S. Kocuria coralli sp. nov., a novel actinobacterium isolated from coral reef seawater. Int J Syst Evol Microbiol 2020; 70:785–789 [View Article][PubMed]
    [Google Scholar]
  18. Xu P, Li W-J, Tang S-K, Zhang Y-Q, Chen G-Z et al. Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. Int J Syst Evol Microbiol 2005; 55:1149–1153 [View Article][PubMed]
    [Google Scholar]
  19. Collins MD, Pirouz T, Goodfellow M, Minnikin DE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 1977; 100:221–230 [View Article][PubMed]
    [Google Scholar]
  20. Groth I, Schumann P, Rainey FA, Martin K, Schuetze B et al. Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. Int J Syst Bacteriol 1997; 47:1129–1133 [View Article][PubMed]
    [Google Scholar]
  21. Minnikin DE, Collins MD, Goodfellow M. Fatty-acid and polar lipid-composition in the classification of Cellulomonas, oerskovia and related taxa. J Appl Bacteriol 1979; 47:87–95 [View Article]
    [Google Scholar]
  22. Collins MD, Jones D. Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. J Appl Microbiol 1980; 48:459–470
    [Google Scholar]
  23. Staneck JL, Roberts GD. Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 1974; 28:226–231 [View Article][PubMed]
    [Google Scholar]
  24. Richter M, Rosselló-Móra R. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci U S A 2009; 106:19126–19131 [View Article][PubMed]
    [Google Scholar]
  25. Thompson CC, Chimetto L, Edwards RA, Swings J, Stackebrandt E et al. Microbial genomic taxonomy. BMC Genom 2013; 14:913 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004460
Loading
/content/journal/ijsem/10.1099/ijsem.0.004460
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error