Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana, respectively. On the basis of phylogenetic analysis based on the combined D1/D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, Lachancea fermentati CBS 707T was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene, the D1/D2 domain of the LSU rRNA gene, the ITS region, translation elongation factor-1 alpha (TEF1) and RNA polymerase II subunit 2 (RPB2) genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence, Savitreea pentosicarens gen. nov., sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is S. pentosicarens DMKU-GTCP10-8T (ex-type strain TBRC 12159=PYCC 8490; MycoBank number 835044).
SakpuntoonV, AngchaunJ, BoonmakC, ChangC-F, LiuS-M et al.Wickerhamiella osmotolerans sp. nov. and Wickerhamiella tropicalis sp. nov., novel ascomycetous yeast in the family Wickerhamiellaceae. Int J Syst Evol Microbiol2020; 70:2589–2594 [View Article][PubMed]
LachanceM-A, ValeHMM, SperandioEM, CarvalhoAOS, SantosARO et al.Wickerhamiella dianesei f.a., sp. nov. and Wickerhamiella kurtzmanii f.a., sp. nov., two yeast species isolated from plants and insects. Int J Syst Evol Microbiol2018; 68:3351–3355 [View Article][PubMed]
JutakanokeR, EndohR, TakashimaM, OhkumaM, TanasupawatS et al.Allodekkera sacchari gen. nov., sp. nov., a yeast species in the Saccharomycetales isolated from a sugar factory. Int J Syst Evol Microbiol2017; 67:250–255 [View Article][PubMed]
BoonthamW, AngchuanJ, BoonmakC, SrisukN. Limtongozyma siamensis gen. nov., sp. nov., a yeast species in the Saccharomycetales and reassignment of Candida cylindracea to the genus Limtongozyma. Int J Syst Evol Microbiol2019
SakpuntoonV, AngchuanJ, BoonthamW, KhunnamwongP, BoonmakC et al. Grease waste as a reservoir of lipase-producing yeast and descReservoir of Lipase-Producing Yeast and Description of Limtongella siamensis gen. nov., sp. nov. Microorganisms2020; 8:
JacquesN, CasaregolaS. Large biodiversity of yeasts in French Guiana and the description of Suhomyces coccinellae f.a. sp. nov. and Suhomyces faveliae f.a. sp. nov. Int J Syst Evol Microbiol2019; 69:1634–1649 [View Article][PubMed]
KurtzmanCP, RobnettCJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie van Leeuwenhoek1998; 73:331–371 [View Article][PubMed]
WhiteTJ, BrunsT, LeeS, TaylorJW, InnisMA et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: A Guide to Methods and Applications1990 pp 315–322
KurtzmanCP, RobnettCJ. Three new anascosporic genera of the Saccharomycotina: Danielozyma gen. nov., Deakozyma gen. nov. and Middelhovenomyces gen. nov. Antonie van Leeuwenhoek2014; 105:933–942 [View Article][PubMed]
VuD, GroenewaldM, SzökeS, CardinaliG, EberhardtU et al. DNA barcoding analysis of more than 9 000 yeast isolates contributes to quantitative thresholds for yeast species and genera delimitation. Stud Mycol2016; 85:91–105 [View Article][PubMed]