1887

Abstract

Two strains (DMKU-GTCP10-8 and CLIB 1740) representing a novel anamorphic yeast species were isolated from a grease sample collected from a grease trap in Thailand and from an unidentified fungus collected in French Guiana, respectively. On the basis of phylogenetic analysis based on the combined D1D2 domain of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, CBS 707 was the closely related species with 12.8 % sequence divergence (70 nucleotide substitutions and three gaps in 571 nucleotides) and 28.1 % sequence divergence (93 nucleotide substitutions and 90 gaps in 651 nucleotides) in the D1/D2 domain of the LSU rRNA gene and the ITS region, respectively. Phylogenetic analysis based on the concatenated sequences of the five genes including the small subunit rRNA gene, the D1D2 domain of the LSU rRNA gene, the ITS region, translation elongation factor-1 alpha () and RNA polymerase II subunit 2 () genes confirmed that the two strains (DMKU-GTCP10-8 and CLIB 1740) were well-separated from other described yeast genera in Saccharomycetaceae. Hence, gen. nov., sp. nov. is proposed to accommodate these two strains as members of the family Saccharomycetaceae. The holotype is DMKUGTCP10-8 (extype strain TBRC 12159PYCC 8490; MycoBank number 835044).

Funding
This study was supported by the:
  • Nantana Srisuk , Faculty of Science, Kasetsart University , (Award International SciKU Branding (ISB))
  • Varunya Sakpuntoon , Thailand Research Fund , (Award the Royal Golden Jubilee PhD program grant no. PHD/0070/2560)
  • Nantana Srisuk , Thailand Research Fund , (Award TRF Research-Team Promotion Grant (RTA608004))
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2020-09-14
2021-03-02
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