RT Journal Article SR Electronic(1) A1 Shen, Ya A1 Chen, Sheng-Chung A1 Lai, Mei-Chin A1 Huang, Hsing-Hua A1 Chiu, Hsiu-Hui A1 Tang, Sen-Lin A1 Rogozin, Denis Yu A1 Degermendzhy, Andrey G.YR 2020 T1 Methanolobus halotolerans sp. nov., isolated from the saline Lake Tus in Siberia JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 10 SP 5586 OP 5593 DO https://doi.org/10.1099/ijsem.0.004453 PB Microbiology Society, SN 1466-5034, AB A halotolerant, psychrotolerant and methylotrophic methanogen, strain SY-01T, was isolated from the saline Lake Tus in Siberia. Cells of strain SY-01T were non-motile, cocci and 0.8–1.0 µm in diameter. The only methanogenic substrate utilized by strain SY-01T was methanol. The temperature range of growth for strain SY-01T was from 4 to 40 °C and the optimal temperature for growth was 30 °C. The pH range of growth was from pH 7.2 to 9.0, with optimal growth at pH 8.0. The NaCl range of growth was 0–1.55 M with optimal growth at 0.51 M NaCl. The G+C content of the genome of strain SY-01T was 43.6 mol % as determined by genome sequencing. Phylogenetic analysis revealed that strain SY-01T was most closely related to Methanolobus zinderi SD1T (97.3 % 16S rRNA gene sequence similarity), and had 95.5–97.2 % similarities to other Methanolobus species with valid names. Genome relatedness between strain SY-01T and DSM 21339T was computed using average nucleotide identity and digital DNA–DNAhybridization, which yielded values of 79.7 and 21.7 %, respectively. Based on morphological, phenotypic, phylogenetic and genomic relatedness data presented here, it is evident that strain SY-01T represents a novel species of the genus Methanolobus , and the name Methanolobus halotolerans sp. nov. is proposed. The type strain is SY-01T (=BCRC AR10051T=NBRC 113166 T=DSM 107642T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004453