1887

Abstract

Two Gram-stain-negative, aerobic, non-motile bacterial strains, 36D10-4-7 and 30C10-4-7, were isolated from bark canker tissue of , respectively. 16S rRNA gene sequence analysis revealed that strain 36D10-4-7 shows 98.0 % sequence similarity to DSM 7418, and strain 30C10-4-7 shows highest sequence similarity to H-12 (95.6 %). Average nucleotide identity analysis indicates that strain 36D10-4-7 is a novel member different from recognized species in the genus . The main fatty acids and respiratory quinone detected in strain 36D10-4-7 are C 7 and/or C 6 and Q-10, respectively. The polar lipids are diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, aminolipid, phosphatidylethanolamine, sphingoglycolipid, two uncharacterized phospholipids and two uncharacterized lipids. For strain 30C10-4-7, the major fatty acids and menaquinone are iso-C, C 7 and/or C 6 and iso-C 3-OH and MK-7, respectively. The polar lipid profile includes phosphatidylethanolamine, phospholipids, two aminophospholipids and six unidentified lipids. Based on phenotypic and genotypic characteristics, these two strains represent two novel species within the genera and . The name sp. nov. (type strain 36D10-4-7=CFCC 13112=KCTC 52799) and sp. nov. (type strain 30C10-4-7=CFCC 13069=KCTC 52797) are proposed.

Funding
This study was supported by the:
  • , National Microbial Resources Collection , (Award NMRC-2020–7)
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2020-09-14
2020-10-20
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