@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004450, author = "Kim, Min Ji and Roh, Su Gwon and Kim, Min-Kyeong and Park, Changwoo and Kim, Seil and Kim, Seung Bum", title = "Kitasatospora acidiphila sp. nov., isolated from pine grove soil, exhibiting antimicrobial potential", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "10", pages = "5567-5575", doi = "https://doi.org/10.1099/ijsem.0.004450", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004450", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "pine grove soil", keywords = "Kitasatospora acidiphila", keywords = "antimicrobial potential", keywords = "biosynthetic gene cluster", abstract = "A polyphasic study was carried out to establish the taxonomic position of an acidophilic isolate designated MMS16-CNU292T (=JCM 32302T) from pine grove soil, and provisionally assigned to the genus Kitasatospora . On the basis of 16S rRNA gene sequence similarity, the strain formed a novel evolutionary lineage within Kitasatospora and showed highest similarities to Kitasatospora azatica KCTC 9699T (98.75 %), Kitasatospora kifunensis IFO 15206T (98.74 %), Kitasatospora purpeofusca NRRL B-1817T (98.61 %) and Kitasatospora nipponensis HKI 0315T (98.42 %), respectively. Strain MMS16-CNU292T possessed MK-9(H6) and MK-9(H8) as the major menaquinones, and a major amount of meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell hydrolysates were rich in galactose, glucose and mannose, and the polar lipids mainly consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannosides. The major fatty acids were anteiso-C15 : 1-A, anteiso-C15 : 0, and iso-C15 : 0, and the DNA G+C content was 71.5 mol%. The strain exhibited antibacterial activity against a number of bacterial strains, and the activity was generally greater when grown in acidic conditions. The phylogenetic, chemotaxonomic and phenotypic properties enabled distinction of MMS16-CNU292T from related species, and thus the isolate should be recognized as a new species of the genus Kitasatospora , for which the name Kitasatospora acidiphila sp. nov. (type strain=MMS16-CNU292T=KCTC 49011T=JCM 32302T) is proposed.", }