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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 10

sp. nov. and sp. nov., isolated from deep-sea sediment of the South China Sea Free

Abstract

Two novel Gram-stain-positive bacteria, designated as SCSIO 52909 and SCSIO 52915, were isolated from a deep-sea sediment sample collected at about 3448 m water depth of the South China Sea. Phenotypic, chemotaxonomic and genomic characteristics were investigated. These strains were aerobic and tested positive for catalase activity, oxidase activity and nitrate reduction. Optimal growth occurred at 28 °C, pH 7 and 3% salinity over 14 days cultivation. Its peptidoglycan structure was type A3α (-Lys-Ala) and the only menaquinone was MK-8. Both strains possessed diphosphatidylglycerol, phosphatidylglycerol, an unidentified phosphoglycolipid, an unidentified glycolipid and an unidentified phospholipid. Their major fatty acids differed, but both contained iso-branched components of C 12-methyl. Genome sequencing revealed two large genomes of 4.58 Mbp with G+C content of 67.0 mol% in SCSIO 52909 and of 4.42 Mbp with G+C content of 69.1 % in SCSIO 52915. The two novel strains encoded genes for metabolism that are absent in most other species, and possessed many more gene copy numbers of alkaline phosphatase and thioredoxin reductase. Results of gANI and 16S rRNA gene analyses suggested that the two strains represent two new species, with 74.9, 95.0 % pairwise similarity between each other, and less than 74.3 and 93.5 % to other recognized species, respectively. In the phylogenetic analysis, strains SCSIO 52909 and SCSIO 52915 were separately clustered together and formed a well-separated phylogenetic branch distinct from the other known species in the genus . Based on the data presented here, these two strains should be recognized as two new species in the genus , for which the names sp. nov., with the type strain SCSIO 52909 (=KCTC 49412=CGMCC 1.13853), and sp. nov., with the type strain SCSIO 52915 (=KCTC 49411=CGMCC 1.13852), are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Marine actinobacteria , polyphasic taxonomy , Rubrobacter marinus sp. nov. , Rubrobacter tropicus sp. nov. and South China Sea
Funding
This study was supported by the:
  • Youth Innovation Promotion Association of the Chinese Academy of Sciences (Award 2016307)
    • Principle Award Recipient: Xin-Peng Tian
  • National Natural Science Foundation of China (Award 41576143)
    • Principle Award Recipient: Xin-Peng Tian
  • Strategic Priority Research Program of the Chinese Academy of Sciences (Award XDA19060301)
    • Principle Award Recipient: Xin-Peng Tian
© 2020 The Authors
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2020-09-17
2025-01-18
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Rubrobacter tropicus sp. nov. and Rubrobacter marinus sp. nov., isolated from deep-sea sediment of the South China Sea
Int J Syst Evol Microbiol 70, 5576 (2020); https://doi.org/10.1099/ijsem.0.004449
/content/journal/ijsem/10.1099/ijsem.0.004449
/content/journal/ijsem/10.1099/ijsem.0.004449
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