1887

Abstract

Two gene-harbouring bacterial strains were isolated from rhizospheres of different vegetable plants grown in different regions of northern PR China. The two strains possessed almost identical 16S rRNA gene sequences. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between the two strains were 99.21 and 93.6% respectively, suggesting they belong to one species. Based on 16S rRNA gene phylogeny, the two strains were clustered together with 7197, T27 and T98, but on a separate branch. Novelty of the species was confirmed by ANI and dDDH comparisons between the type strain 7124 and its closest relatives, since the obtained values were considerably below the proposed thresholds for the species delineation. The genome size of strain 7124 was 5.40 Mb, comprising 5050 predicted genes with a DNA G+C content of 52.3 mol%. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified lipids. The major cellular fatty acids were anteiso-C (52.9%) and C (23.4 %). Menaquinone-7 was reported as the major respiratory quinone. The diamino acid in the cell-wall peptidoglycan was found to be -diaminopimelic acid. Based on phylogenetic, genomic, chemotaxonomic and phenotypic data, the two isolates are considered to represent a novel species of the genus , for which the name sp. nov. is proposed, with 7124 (=DSM 103172=CGMCC 1.15689) as type strain.

Funding
This study was supported by the:
  • Jun-lian Gao , Science & Technology Innovation Project of Beijing Academy of Agricultural and Forestry Sciences , (Award KJCX20200519)
  • Jian-guang Sun , the National Natural Science Foundation of China , (Award No. 31870003)
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2020-09-11
2020-11-30
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