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Abstract

Strain aSej3 was isolated from a root nodule of a plant growing in Bizerte, Tunisia. 16S rRNA gene analysis placed this strain within the genus . Multilocus sequence analysis (MLSA) including three housekeeping genes (, and ) grouped aSej3 together with CTAW71, CTAW11, RITF806, USDA 3051 and BTA-1. MLSA with five housekeeping genes (, , , and ) revealed that this strain shares less than 93.5 % nucleotide identity with other type strains. Genome sequencing and inspection revealed a genome size of 8.83 Mbp with a G+C content of 62.8 mol%. Genome-wide average nucleotide identity and digital DNA–DNA hybridization values were below 87.5 and 36.2 %, respectively, when compared to described species. Strain aSej3 nodulated plants under axenic conditions and its gene clustered within the genistearum symbiovar. Altogether, the phylogenetic data and the chemotaxonomic characteristics of this strain support that aSej3 represents a new species for which we propose the name sp. nov. with the type strain aSej3 (=DSM 108913=LMG 31020).

Funding
This study was supported by the:
  • Bundesministerium für Bildung und Forschung (Award 01DH16008)
    • Principle Award Recipient: Not Applicable
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2020-09-08
2024-03-29
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