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Volume 70, Issue 10

sp. nov., a methanotroph isolated from a geothermal field in the Republic of Korea Free

Abstract

A Gram-stain-negative, aerobic, non-motile and coccoid methanotroph, strain IM1, was isolated from hot spring soil. Cells of strain IM1 were catalase-negative, oxidase-positive and displayed a characteristic intracytoplasmic membrane arrangement of type I methanotrophs. The strain possessed genes encoding both membrane-bound and soluble methane monooxygenases and grew only on methane or methanol. The strain was capable of growth at temperatures between 15 and 48 °C (optimum, 30–45 °C) and pH values between pH 4.8 and 8.2 (optimum, pH 6.2–7.0). Based on phylogenetic analysis of 16S rRNA gene and PmoA sequences, strain IM1 was demonstrated to be affiliated to the genus . The 16S rRNA gene sequence of this strain was most closely related to the sequences of an uncultured bacterium clone FD09 (100 %) and a partially described cultured sp. GDS2.4 (99.78 %). The most closely related taxonomically described strains were Texas (97.92 %), Bath (97.86 %) and 73a (94.21 %). Strain IM1 shared average nucleotide identity values of 85.93 and 85.62 % with strains Texas and Bath, respectively. The digital DNA–DNA hybridization value with the closest type strain was 29.90 %. The DNA G+C content of strain IM1 was 63.3 mol% and the major cellular fatty acids were C (39.0 %), C 7 (24.0 %), C 6 (13.6 %) and C 5 (12.0 %). The major ubiquinone was methylene-ubiquinone-8. On the basis of phenotypic, genetic and phylogenetic data, strain IM1 represents a novel species of the genus for which the name sp. nov. is proposed, with strain IM1 (=JCM 33941=KCTC 72677) as the type strain.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): methane , methanotroph , Methylococcus and thermotolerant
Funding
This study was supported by the:
  • Ministry of Oceans and Fisheries, Korea (Award 20170411)
    • Principle Award Recipient: Not Applicable
  • National Institute of Biological Resources (Award NIBR201902111)
    • Principle Award Recipient: Sung-Keun Rhee
  • National Research Foundation (Award 2015M3D3A1A01064881)
    • Principle Award Recipient: Sung-Keun Rhee
© 2020 The Authors
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2020-09-10
2024-04-23
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Methylococcus geothermalis sp. nov., a methanotroph isolated from a geothermal field in the Republic of Korea
Int J Syst Evol Microbiol 70, 5520 (2020); https://doi.org/10.1099/ijsem.0.004442
/content/journal/ijsem/10.1099/ijsem.0.004442
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