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Abstract

A Gram-stain-negative, non-motile, coccus-shaped, catalase- and oxidase-positive, facultatively anaerobic and pink-pigmented bacterium, designated strain CQN31, was isolated from sediment of Changqiaohai Lake, Yunnan Province, China. Growth occurred at 4–45 °C (optimum, 37 °C), at pH 6.5–9.5 (optimum, pH 8.0) and with 0–1 % (w/v) NaCl (optimum, 0 %). C 7/C 6 and C were the predominant fatty acids. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidyldimethylethanolamine (PME) and one unidentified aminolipid (AL) were the major polar lipids. The G+C content of the genomic DNA was 71.5 %. 16S rRNA gene sequence comparisons indicated that strain CQN31 shared 96.8 % similarity with JCM 19527 and 95.9 % with EM0302. Digital DNA–DNA hybridization values between strain CQN31 and DSM 19981, DSM 14916 and NCTC 13291 were 21.0, 19.4 and 19.8 %, respectively. Average amino acid identity and average nucleotide identity values between strain CQN31 and DSM 19981, DSM 14916 and NCTC 13291 were 73.7, 63.4 and 61.9 %, and 79.2, 77.1 and 77.5%, respectively. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain CQN31 as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CQN31 (=KCTC 62447=MCCC 1H00309).

Funding
This study was supported by the:
  • the Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: Dashuai Mu
  • the National Natural Science Foundation of China (Award 41876166)
    • Principle Award Recipient: Dashuai Mu
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2020-09-04
2021-08-02
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