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Volume 70, Issue 10

sp. nov., isolated from lake sediment Free

Abstract

A Gram-stain-negative, non-motile, coccus-shaped, catalase- and oxidase-positive, facultatively anaerobic and pink-pigmented bacterium, designated strain CQN31, was isolated from sediment of Changqiaohai Lake, Yunnan Province, China. Growth occurred at 4–45 °C (optimum, 37 °C), at pH 6.5–9.5 (optimum, pH 8.0) and with 0–1 % (w/v) NaCl (optimum, 0 %). C 7/C 6 and C were the predominant fatty acids. Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidyldimethylethanolamine (PME) and one unidentified aminolipid (AL) were the major polar lipids. The G+C content of the genomic DNA was 71.5 %. 16S rRNA gene sequence comparisons indicated that strain CQN31 shared 96.8 % similarity with JCM 19527 and 95.9 % with EM0302. Digital DNA–DNA hybridization values between strain CQN31 and DSM 19981, DSM 14916 and NCTC 13291 were 21.0, 19.4 and 19.8 %, respectively. Average amino acid identity and average nucleotide identity values between strain CQN31 and DSM 19981, DSM 14916 and NCTC 13291 were 73.7, 63.4 and 61.9 %, and 79.2, 77.1 and 77.5%, respectively. Distinct morphological, physiological and genotypic differences from previously described taxa support the classification of strain CQN31 as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CQN31 (=KCTC 62447=MCCC 1H00309).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): draft genome sequencing , prokaryotic taxonomy and Roseomonas bella
Funding
This study was supported by the:
  • the Science and Technology Basic Resources Investigation Program of China (Award 2017FY100300)
    • Principle Award Recipient: Dashuai Mu
  • the National Natural Science Foundation of China (Award 41876166)
    • Principle Award Recipient: Dashuai Mu
© 2020 The Authors
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2020-09-04
2024-04-25
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References

  1. Han XY, Pham AS, Tarrand JJ, Rolston KV, Helsel LO et al. Bacteriologic characterization of 36 strains of Roseomonas species and proposal of Roseomonas mucosa sp nov and Roseomonas gilardii subsp rosea subsp nov. Am J Clin Pathol 2003; 120:256–264 [View Article][PubMed]
     [Google Scholar]
  2. Subudhi CP, Adedeji A, Kaufmann ME, Lucas GS, Kerr JR. Fatal Roseomonas gilardii bacteremia in a patient with refractory blast crisis of chronic myeloid leukemia. Clin Microbiol Infect 2001; 7:573–575 [View Article][PubMed]
     [Google Scholar]
  3. Bibashi E, Sofianou D, Kontopoulou K, Mitsopoulos E, Kokolina E. Peritonitis due to Roseomonas fauriae in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 2000; 38:456–457[PubMed]
     [Google Scholar]
  4. Sandoe JA, Malnick H, Loudon KW. A case of peritonitis caused by Roseomonas gilardii in a patient undergoing continuous ambulatory peritoneal dialysis. J Clin Microbiol 1997; 35:2150–2152 [View Article][PubMed]
     [Google Scholar]
  5. Rihs JD, Brenner DJ, Weaver RE, Steigerwalt AG, Hollis DG et al. Roseomonas, a new genus associated with bacteremia and other human infections. J Clin Microbiol 1993; 31:3275–3283 [View Article][PubMed]
     [Google Scholar]
  6. Lee Y, Jeon CO. Roseomonas aeriglobus sp. nov., isolated from an air-conditioning system. Antonie van Leeuwenhoek 2018; 111:343–351 [View Article][PubMed]
     [Google Scholar]
  7. Subhash Y, Lee S-S. Roseomonas deserti sp. nov., isolated from crude oil contaminated desert sand. Int J Syst Evol Microbiol 2018; 68:675–680 [View Article][PubMed]
     [Google Scholar]
  8. Ko Y, Yim J, Hwang WM, Kang K, Ahn T-Y. Roseomonas fluminis sp. nov. isolated from sediment of a shallow stream. Int J Syst Evol Microbiol 2018; 68:782–787 [View Article][PubMed]
     [Google Scholar]
  9. Fang X-M, Bai J-L, Zhang D-W, Su J, Zhao L-L et al. Roseomonas globiformis sp. nov., an airborne bacteria isolated from an urban area of Beijing. Int J Syst Evol Microbiol 2018; 68:3301–3306 [View Article][PubMed]
     [Google Scholar]
  10. Kim J-Y, Kim D-U, Kang M-S, Jang JH, Kim SJ et al. Roseomonas radiodurans sp. nov., a gamma-radiation-resistant bacterium isolated from gamma ray-irradiated soil. Int J Syst Evol Microbiol 2018; 68:2443–2447 [View Article][PubMed]
     [Google Scholar]
  11. Liu Q-Q, Wang Y, Li J, Du Z-J, Chen G-J. Saccharicrinis carchari sp. nov., isolated from a shark, and emended descriptions of the genus Saccharicrinis and Saccharicrinis fermentans . Int J Syst Evol Microbiol 2014; 64:2204–2209 [View Article][PubMed]
     [Google Scholar]
  12. Li R, Zhu H, Ruan J, Qian W, Fang X et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res 2010; 20:265–272 [View Article][PubMed]
     [Google Scholar]
  13. Angiuoli SV, Gussman A, Klimke W, Cochrane G, Field D et al. Toward an online repository of Standard Operating Procedures (SOPs) for (meta)genomic annotation. OMICS 2008; 12:137–141 [View Article][PubMed]
     [Google Scholar]
  14. Kanehisa M, Sato Y, Kawashima M, Furumichi M, Tanabe M. KEGG as a reference resource for gene and protein annotation. Nucleic Acids Res 2016; 44:D457–D462 [View Article][PubMed]
     [Google Scholar]
  15. Kanehisa M, Sato Y, Morishima K. BlastKOALA and GhostKOALA: KEGG tools for functional characterization of genome and metagenome sequences. J Mol Biol 2016; 428:726–731 [View Article][PubMed]
     [Google Scholar]
  16. Yoon S-H, Ha S-M, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 2017; 110:1281–1286 [View Article][PubMed]
     [Google Scholar]
  17. Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [View Article][PubMed]
     [Google Scholar]
  18. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
     [Google Scholar]
  19. Felsenstein J, Joseph F. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
     [Google Scholar]
  20. Bowman JP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol 2000; 50 Pt 5:1861–1868 [View Article][PubMed]
     [Google Scholar]
  21. Wang N-N, Liu Z-Y, Jiang L-X, Li Y-X, Du Z-J et al. Roseovarius salinarum sp. nov., isolated from a marine solar saltern. Int J Syst Evol Microbiol 2018; 68:1986–1991 [View Article][PubMed]
     [Google Scholar]
  22. CLSI Performance standards for antimicrobial susceptibility testing; Twenty-second Informational supplement. CLSI document M100-S22 Wayne PA: Clinical and Laboratory Standards Institute; 2013
     [Google Scholar]
  23. Kroppenstedt RM. Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 1982; 5:2359–2367 [View Article]
     [Google Scholar]
  24. Fang D-B, Han J-R, Liu Y, Du Z-J. Seonamhaeicola marinus sp. nov., isolated from marine algae. Int J Syst Evol Microbiol 2017; 67:4857–4861 [View Article][PubMed]
     [Google Scholar]
  25. Lee JH, Kim MS, Baik KS, Kim HM, Lee KH et al. Roseomonas wooponensis sp. nov., isolated from wetland freshwater. Int J Syst Evol Microbiol 2015; 65:4049–4054 [View Article][PubMed]
     [Google Scholar]
  26. Damtab J, Nutaratat P, Boontham W, Srisuk N, Duangmal K et al. Roseomonas elaeocarpi sp. nov., isolated from olive (Elaeocarpus hygrophilus Kurz.) phyllosphere. Int J Syst Evol Microbiol 2016; 66:474–480 [View Article][PubMed]
     [Google Scholar]
  27. Kim D-U, Lee H, Kim S-G, Ka J-O. Roseomonas terricola sp. nov., isolated from agricultural soil. Int J Syst Evol Microbiol 2017; 67:4836–4841 [View Article][PubMed]
     [Google Scholar]
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Roseomonas bella sp. nov., isolated from lake sediment
Int J Syst Evol Microbiol 70, 5473 (2020); https://doi.org/10.1099/ijsem.0.004436
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