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Abstract

A novel Gram-stain-positive, non-motile, non-spore-forming, coccobacillus-shaped, strictly aerobic bacterium, designated strain H23T48, was isolated from the faecal sample of an oriental stork collected from the Seoul Grand Park Zoo in Seoul, Republic of Korea. Optimal growth of strain H23T48 was observed at 30–37 °C, pH 8 and with 3 % (w/v) NaCl. 16S rRNA gene sequence-based phylogenetic analysis revealed that strain H23T48 was closely related to the genus , with 97.0 and 96.7 % sequence similarities to EBR4-1-2 and H5, respectively. Strain H23T48 possessed MK-9(H) as the major menaquinone and C (42.4 %), C 9 (31.3 %) and C (17.7 %) as the major cellular fatty acids. The polar lipids included phosphatidylglycerol, two unidentified lipids, six unidentified phospholipids and two unidentified glycophospholipids. The amino acid composition of the cell-wall peptidoglycan was alanine, lysine, glutamic acid, aspartic acid and glycine. The genomic G+C content of strain H23T48 is 59.5 mol% and the average nucleotide identity value between H23T48 and KCT C33148 (=EBR4-1-2) is 75.5 %. Based on the obtained data, strain H23T48 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is H23T48 (=KCTC 49253=JCM 33282).

Funding
This study was supported by the:
  • Jin-Woo Bae , National Research Foundation of Korea , (Award NRF-2020R1A2C3012797)
  • Jin-Woo Bae , National Research Foundation of Korea , (Award NRF-2018R1A5A1025077)
  • Jin-Woo Bae , Ministry of Food and Drug Safety , (Award 20172MFDS195)
  • Jin-Woo Bae , Ministry of Environment (MOE) of the Republic of Korea , (Award NIBR201801106)
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/content/journal/ijsem/10.1099/ijsem.0.004435
2020-09-04
2020-09-28
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