@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004434, author = "Lenchi, Nesrine and Anzil, Adriana and Servais, Pierre and Kebbouche-Gana, Salima and Gana, Mohamed Lamine and Llirós, Marc", title = "Microbacterium algeriense sp. nov., a novel actinobacterium isolated from Algerian oil production waters", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "12", pages = "6044-6051", doi = "https://doi.org/10.1099/ijsem.0.004434", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004434", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "oilfield", keywords = "actinobacteria", keywords = "Microbacterium algeriense sp. nov.", keywords = "production water", abstract = "A non-motile, straight-rod-shaped, Gram-stain-positive and facultative anaerobic bacterium (i.e., strain G1T) was isolated from production waters from an Algerian oilfield. Growth was observed in the presence of 0.3–3.5 % (w/v) NaCl, at 20–50 °C and at pH 6.0–9.0. Results of phylogenetic analyses based on 16S rRNA gene sequences showed that strain G1T belonged to the genus Microbacterium . Strain G1 T was closely related to Microbacterium oxydans (DSM 20578T) and Microbacterium maritypicum (DSM 12512T) with 99.8 % sequence similarity and to Microbacterium saperdae (DSM 20169T) with 99.6 % sequence similarity. Strain G1 T contained MK9, MK10, MK11, MK12 and MK13 as respiratory quinones, and phosphatidylglycerol, diphosphatidylglycerol and glycolipid as the major polar lipids. The major cellular fatty acids were anteiso-C15:0, iso-C16:0 and anteiso-C17:0. The estimated DNA G+C content was 69.57 mol% based on its draft genome sequence. Genome annotation of strain G1T predicted the presence of 3511 genes, of which 3483 were protein-coding and 47 were tRNA genes. The DNA–DNA hybridization (DDH) and average nucleotide identity (ANI) values between strain G1T and M. oxydans (DSM 20578T) and M. maritypicum (DSM 12512T) were in both cases far below the respective species boundary thresholds (27.5 and 28.0 % for DDH; and 84.40 and 84.82% for ANI, respectively). Based on the data presented above, strain G1T was considered to represent a novel species for which the name Microbacterium algeriense is proposed with the type strain G1T (=DSM 109018T=LMG 31276T).", }