1887

Abstract

A novel actinomycete strain, CT2-14, belonging to the genus , was isolated from a soil sample collected from Phichit Province, Thailand. The taxonomic position of the strain was characterized using a polyphasic approach. The strain grew at 15–40 °C (optimum, 28–37 °C), pH 6–11 (optimum, pH 6–8) and on an International Project 2 with 4 % (w/v) NaCl agar plate. -diaminopimelic acid was detected in the cell-wall peptidoglycan. Ribose, arabinose and galactose were detected in its whole-cell hydrolysates. Mycolic acids were present. The strain contained C, summed feature 3, C 10-methyl and C 9 as the major fatty acids and MK-8(Hω-cycl) as the major menaquinone. The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol and phosphatidylinositol mannosides. Strain CT2-14 showed the highest 16S rRNA gene similarity to JCM 11307 (98.4 %), JCM 11438 (98.2 %) and JCM 13032 (98.0 %). The draft genome of strain CT2-14 was 7.37 Mb with 6685 coding sequences with an average G+C content of 67.9 mol %. Based on the phylogenomic tree analysis, the strain was closely related to NBRC 100131. On the basis of polyphasic and genome analyses, strain CT2-14 represented a novel species of the genus for which the name sp. nov. is proposed. The type strain is CT2-14 (=JCM 33775=TISTR 2838).

Funding
This study was supported by the:
  • Ratchadapiseksomphot Endowment Fund, Chulalongkorn University for a post-doctoral fellowship
    • Principle Award Recipient: Pawina Kanchanasin
  • Grant for International Research Integration, Research Pyramid, Ratchadaphiseksomphot Endowment Fund, Chulalongkorn University (Award GCURP_58_01_33_01)
    • Principle Award Recipient: Somboon Tanasupawat
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2020-09-08
2021-10-24
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