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Abstract

An actinobacterial strain, designated KUDC0627, was isolated from rhizospheric soil that contained on the Dokdo Islands, Republic of Korea. Cells were Gram-stain-positive, facultative anaerobic, non-motile and non-endospore-forming cocci. Results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain KUDC0627 belongs to the genus and is most closely related to DSM 21800 (98.5 %), DSM 100019 (97.7 %) and Gsoil 633 (96.5 %). The average nucleotide identity scores and average amino acid identity values were all below the 95.0 % cut-off point. DNA–DNA hybridization, using the Genome-to-Genome Distance Calculator, estimated that there is 22.3 % DNA relatedness between KUDC0627 and DSM 21800. The genomic DNA G+C content was 66.9 mol%. The major menaquinone was MK-9(H) and the major diagnostic diamino acid in the cell-wall peptidoglycan was -diaminopimelic acid. The polar lipid profile included diphosphatidylglycerol, phosphatidylglycerol, unidentified phospholipids, unidentified glycolipids and unidentified lipids. The major cellular fatty acids were iso-C, anteiso-C and iso-C. Based on phenotypic, chemotaxonomic, and phylogenetic data, strain KUDC0627 (=KCTC 39853=JCM 32702) represents a novel species, for which the name sp. nov. is proposed.

Funding
This study was supported by the:
  • Sa-Youl Ghim , Ministry of Education (KR) , (Award 2016R1A05011910)
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/content/journal/ijsem/10.1099/ijsem.0.004428
2020-09-04
2020-09-22
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