1887

Abstract

Strain HB172011 was isolated from mangrove soil sampled at the Bamenbay mangrove forest, PR China. Cells were easily recognized under the microscope as cocci that were usually arranged in distinctive tetrads. Results of phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate belongs to the genus and has 95.6–96.3% 16S rRNA gene sequence similarities to the four type strains. The strain was aerobic or facultatively anaerobic, Gram-stain-negative and non-motile. Cells were found to grow at 10–40 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, pH 7.0) and with 0–9.0% (w/v) NaCl (optimum, 2–4%). Major fatty acids were feature 8 (C 7 and/or C 6), C, C cyclo 8 and summed feature 2 (C iso I and/or C–3 OH). Genome sequencing revealed a genome size of 4.87 Mbp and a DNA G+C content of 69.9 mol %. Based on these data, strain HB172011 represents a novel species of , for which the name sp. nov. is proposed. The type strain is HB172011 (=CGMCC 1.16728=JCM 33334).

Funding
This study was supported by the:
  • Financial Fund of the Ministry of Agriculture and Rural Affairs of China (Award NFZX2018)
    • Principle Award Recipient: Huiqin Huang
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004420
2020-08-27
2024-10-06
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/70/10/5389.html?itemId=/content/journal/ijsem/10.1099/ijsem.0.004420&mimeType=html&fmt=ahah

References

  1. Garrity GM, Bell JA, Lilburn T. Family I. Rhodobacteraceae fam. nov. In Brenner DJ, Krieg NR, Staley JT, Garrity GM. (editors) Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 2 (The Proteobacteria), part C (The Alpha-, Beta-, Delta-, and Epsilonproteobacteria) New York: Springer; 2005 p 161
    [Google Scholar]
  2. Maszenan AM, Seviour RJ, Patel BK, Rees GN, McDougall BM. Amaricoccus gen. nov., a Gram-negative coccus occurring in regular packages or tetrads, isolated from activated sludge biomass, and descriptions of Amaricoccus veronensis sp. nov., Amaricoccus tamworthensis sp. nov., Amaricoccus macauensis sp. nov., and Amaricoccus kaplicensis sp. nov. Int J Syst Bacteriol 1997; 47:727–734 [View Article][PubMed]
    [Google Scholar]
  3. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article][PubMed]
    [Google Scholar]
  4. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article][PubMed]
    [Google Scholar]
  5. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article][PubMed]
    [Google Scholar]
  6. Guindon S, Gascuel O. A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 2003; 52:696–704 [View Article][PubMed]
    [Google Scholar]
  7. Rzhetsky A, Nei M. A simple method for estimating and testing minimum evolution trees. Mol Biol Evol 1992; 9:945–967
    [Google Scholar]
  8. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article][PubMed]
    [Google Scholar]
  9. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [View Article][PubMed]
    [Google Scholar]
  10. Brettin T, Davis JJ, Disz T, Edwards RA, Gerdes S et al. RASTtk: a modular and extensible implementation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci Rep 2015; 5:8365–8370 [View Article][PubMed]
    [Google Scholar]
  11. Bhatt HB, Azmatunnisa Begum M, Chintalapati S, Chintalapati VR, Singh SP. Desertibacillus haloalkaliphilus gen. nov., sp. nov., isolated from a saline desert. Int J Syst Evol Microbiol 2017; 67:4435–4442 [View Article][PubMed]
    [Google Scholar]
  12. Sasser M. Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 2001; 101:
    [Google Scholar]
  13. Komagata K, Suzuki KI. Lipids and cell-wall analysis in bacterial systematics. Methods Microbiol 198; 19:161–207
    [Google Scholar]
  14. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
/content/journal/ijsem/10.1099/ijsem.0.004420
Loading
/content/journal/ijsem/10.1099/ijsem.0.004420
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error