sp. nov. and sp. nov., two new members of the genus Free

Abstract

Four unknown strains belonging to the genus were isolated from plateau wildlife on the Qinghai–Tibet Plateau of PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that the four isolates were separated into two clusters. Cluster I (strains 785 and 208) had the greatest 16S rRNA gene sequence similarity to (98.6 and 98.7 %, respectively), (98.0 and 98.1%, respectively), (97.9 and 98.0 %, respectively) and (97.6 and 97.7 %, respectively). Likewise, cluster II (strains J391 and J915) had the highest sequence similarity to (98.6 and 98.3 %, respectively) and (98.1 and 97.9  %, respectively). Average nucleotide identity and the digital DNA–DNA hybridization values illustrated that the two type strains, 785 and J391, represented two separate novel species that are distinct from all currently recognized species in the genus . These strains had DNA G+C contents of 66.0–66.1 mol% (cluster I) and 68.0 mol% (cluster II). The chemotaxonomic properties of strains 785 and J391 were in line with those of the genus : anteiso-C(79.3 and 40.8 %, respectively) as the major cellular fatty acid, MK-8(H) (65.8 %) or MK-9(H) (75.6 %) as the predominant respiratory quinone, a polar lipid profile comprising diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, glycolipids and phospholipid, and A3 or A4 as the cell wall peptidoglycan type. On the basis of our results, two novel species in the genus are proposed, namely sp. nov. (type strain, 785=CGMCC 1.16725=GDMCC 1.1592=JCM 33491) and sp. nov. (type strain, J391=CGMCC 1.17382=GDMCC 1.1667=JCM 33841).

Funding
This study was supported by the:
  • National Key R&D Program of China (Award 2019YFC1200505)
    • Principle Award Recipient: Jing Yang
  • National Key R&D Program of China (Award 2019YFC1200500)
    • Principle Award Recipient: Jing Yang
  • Research Units of Discovery of Unknown Bacteria and Function (Award 2018RU010)
    • Principle Award Recipient: Jianguo Xu
  • Sanming Project of Medicine in Shenzhen (Award SZSM201811071)
    • Principle Award Recipient: Jianguo Xu
  • National Science and Technology Major Project of China (Award 2018ZX10712001-018)
    • Principle Award Recipient: Shan Lu
  • National Science and Technology Major Project of China (Award 2018ZX10712001-007)
    • Principle Award Recipient: Jing Yang
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2020-08-25
2024-03-29
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