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Abstract

A psychrotolerant actinobacterium, designated strain J5903, was isolated from an alkaline soil sample from the rhizosphere of collected in desertification land surrounding Jiuliancheng Nur in Hebei Province, PR China. Cells of the isolate were Gram-stain-positive, aerobic, non-motile and non-spore-forming cocci. Strain J5903 grew optimally at 20‒25 °C, at pH 7.0‒7.5 and with <1 % (w/v) NaCl. The cell-wall peptidoglycan type was B2γ with -2,4-diaminobutyric acid and -2,4-diaminobutyric acid as diagnostic amino acids. The muramyl residue was acetyl type. The menaquinones were MK-11, MK-12, MK-10 and MK-13. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and one unidentified glycolipid. The major whole-cell fatty acids were anteiso-C, iso-C and anteiso-C. The genomic DNA G+C content was 69.1 mol%. It shared the highest average nucleotide identity and digital DNA–DNA hybridization values with 13S1-3. Phylogenies based on genome sequence showed that strain J5903 and 13S1-3 formed a robust cluster with high bootstrap support. Strain J5903 shared typical chemotaxonomic characteristics with 13S1-3. Combining the polyphasic taxonomic evidence, strain J5903 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is J5903 (=DSM 101894=CGMCC 1.15523).

Funding
This study was supported by the:
  • Xiao-Jun Li , PhD Start-up Foundation of Hebei North University
  • Cheng-Hang Sun , Sino-Hungarian Bilateral S&T Cooperation Projects from 2019 to 2020 , (Award Chinese Project: 8-11; Hungarian project: 2018-2.1.14-TÉT-CN-2018-00021)
  • Xiao-Jun Li , Natural Science Foundation of Hebei Province , (Award C2017405031)
  • Xiao-Jun Li , National Natural Science Foundation of China , (Award 81703476)
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2020-08-24
2021-02-26
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