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Volume 70, Issue 10

sp. nov. and sp. nov., isolated from the gut of the fungus growing-termite Open Access

Abstract

The taxonomic positions of two novel aerobic, Gram-positive actinobacteria, designated strains RB29 and RB68, were determined using a polyphasic approach. Based on 16S rRNA gene sequence analysis, the closest phylogenetic neighbours of RB29 were identified as DSM 102126 (99.2 % similarity) and DSM 43919 (98.7 %), and for strain RB68 was DSM 44148 (98.3 %). Digital DNA–DNA hybridization (dDDH) between RB29 and its closest phylogenetic neighbours, DSM 102126 and DSM 43919, resulted in similarity values of 53.2 % (50.6–55.9 %) and 26.4 % (24.1–28.9 %), respectively. Additionally, the average nucleotide identity (ANI) was 93.2 % (94.0 %) for DSM 102126 and 82.3 % (78.9 %) for DSM 43919. dDDH analysis between strain RB68 and DSM 44148 gave a similarity value of 24.5 % (22.2–27.0 %). Both strains, RB29 and RB68, revealed morphological characteristics and chemotaxonomic features typical for the genus , such as the presence of -diaminopimelic acid in the cell wall, galactose and glucose as major sugar components within whole-cell hydrolysates and the absence of mycolic acids. The major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. Predominant menaquinones were MK-9(H) and MK-9(H) for RB29 and MK-9(H) and MK-9(H) for RB68. The main fatty acids were identified as 10-methyloctadecanoic acid (10-methyl C), 14-methylpentadecanoic acid (iso-C), hexadecanoic acid (C) and -9-octadecanoic acid (Cω9). Here, we propose two novel species of the genus : sp. nov. with the type strain RB29 (=CCUG 72668=NRRL B-65537) and sp. nov. with the type strain RB68 (=CCUG 72669=NRRL B-65538).

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  • Accepted:
  • Published Online:
Keyword(s): Actinomadura , Macrotermes natalensis , new natural products and termite gut
Funding
This study was supported by the:
  • Daimler und Benz Stiftung (Award Beemelmanns)
    • Principle Award Recipient: Christine Beemelmanns
  • Boehringer Ingelheim Stiftung (Award Travel Award)
    • Principle Award Recipient: René Benndorf
  • Deutsche Forschungsgemeinschaft (Award CRC 1127, BE-4799/3-1)
    • Principle Award Recipient: Christine Beemelmanns
© 2020 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2020-08-26
2024-04-25
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Actinomadura rubteroloni sp. nov. and Actinomadura macrotermitis sp. nov., isolated from the gut of the fungus growing-termite Macrotermes natalensis
Int J Syst Evol Microbiol 70, 5255 (2020); https://doi.org/10.1099/ijsem.0.004403
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