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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 10

sp. nov. and sp. nov., isolated from natural salt marsh sediment on the Tibetan Plateau Free

Abstract

Two novel Gram-stain-negative, aerobic and non-motile rods bacteria, designated TQ8S and ZH2S, were isolated from salt marsh sediment collected from the Tibetan Plateau. Strain TQ8S was found to grow at 10–40 °C (optimum, 30 °C), pH 6.0–11.0 (optimum, pH 8.0–9.0) and in the presence of 2–12 % (w/v) NaCl (optimum, 6–8 %). Strain ZH2S was found to grow at 15–40 °C (optimum, 30 °C), pH 7.0–10.0 (optimum pH 9.0) and in the presence of 2–10 % (w/v) NaCl (optimum, 4–6 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strains TQ8S and ZH2S shared 99.07 % sequence similarity between each other and were affiliated with the genus , sharing 97.48 % and 97.41 % of sequence similarity to their closest neighbour Esulfide1, respectively. DNA–DNA hybridization analyses showed 61.0 % relatedness between strains TQ8S and ZH2S. The average nucleotide identity and the average amino acid identity values between the two genomes were 92.33 and 92.84 %, respectively. The values between the two strains and their close phylogenetic relatives were all below 95 %. The major respiratory quinones of strain TQ8S were Q-9 and Q-8, while that of ZH2S was Q-9. The main fatty acids shared by the two strains were C 6c and/or C 7c, C 6c and/or C 7c, C and C 3-OH. Strain ZH2S can be distinguished from TQ8S by a higher proportion of C cyclo 8. The G+C content of the genomic DNA of strains TQ8S and ZH2S were 57.20 and 57.14 mol%, respectively. On the basis of phenotypic distinctiveness and phylogenetic divergence, the two isolates are considered to represent two novel species of the genus , for which the names sp. nov (type strain TQ8S=KCTC 62530=CICC 24572) and sp. nov (type strain ZH2S=KCTC 62531=CICC 24505) are proposed.

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Keyword(s): Halomonas , polyphasic taxonomy , salt marsh and sediment
Funding
This study was supported by the:
  • Youth Innovation Promotion Association of the Chinese Academy of Sciences, http://dx.doi.org/10.13039/501100004739 (Award 2014273)
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 31722008)
  • the Science & Technology Basic Resources Investigation Program of China (Award 2017FY100300)
  • the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (Award 2019QZKK0503)
© 2020 The Authors
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2020-08-20
2024-12-13
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Halomonas rituensis sp. nov. and Halomonas zhuhanensis sp. nov., isolated from natural salt marsh sediment on the Tibetan Plateau
Int J Syst Evol Microbiol 70, 5217 (2020); https://doi.org/10.1099/ijsem.0.004395
/content/journal/ijsem/10.1099/ijsem.0.004395
/content/journal/ijsem/10.1099/ijsem.0.004395
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