1887

Abstract

Two novel Gram-stain-negative, aerobic and non-motile rods bacteria, designated TQ8S and ZH2S, were isolated from salt marsh sediment collected from the Tibetan Plateau. Strain TQ8S was found to grow at 10–40 °C (optimum, 30 °C), pH 6.0–11.0 (optimum, pH 8.0–9.0) and in the presence of 2–12 % (w/v) NaCl (optimum, 6–8 %). Strain ZH2S was found to grow at 15–40 °C (optimum, 30 °C), pH 7.0–10.0 (optimum pH 9.0) and in the presence of 2–10 % (w/v) NaCl (optimum, 4–6 %). Phylogenetic analysis based on the 16S rRNA gene sequences showed that strains TQ8S and ZH2S shared 99.07 % sequence similarity between each other and were affiliated with the genus , sharing 97.48 % and 97.41 % of sequence similarity to their closest neighbour Esulfide1, respectively. DNA–DNA hybridization analyses showed 61.0 % relatedness between strains TQ8S and ZH2S. The average nucleotide identity and the average amino acid identity values between the two genomes were 92.33 and 92.84 %, respectively. The values between the two strains and their close phylogenetic relatives were all below 95 %. The major respiratory quinones of strain TQ8S were Q-9 and Q-8, while that of ZH2S was Q-9. The main fatty acids shared by the two strains were C 6c and/or C 7c, C 6c and/or C 7c, C and C 3-OH. Strain ZH2S can be distinguished from TQ8S by a higher proportion of C cyclo 8. The G+C content of the genomic DNA of strains TQ8S and ZH2S were 57.20 and 57.14 mol%, respectively. On the basis of phenotypic distinctiveness and phylogenetic divergence, the two isolates are considered to represent two novel species of the genus , for which the names sp. nov (type strain TQ8S=KCTC 62530=CICC 24572) and sp. nov (type strain ZH2S=KCTC 62531=CICC 24505) are proposed.

Funding
This study was supported by the:
  • Youth Innovation Promotion Association of the Chinese Academy of Sciences, http://dx.doi.org/10.13039/501100004739 (Award 2014273)
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 31722008)
  • the Science & Technology Basic Resources Investigation Program of China (Award 2017FY100300)
  • the Second Tibetan Plateau Scientific Expedition and Research (STEP) program (Award 2019QZKK0503)
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2020-08-20
2021-10-16
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