%0 Journal Article %A Mao, Haiyan %A Wei, Yuli %A Gao, Yuxin %A Pei, Jiahao %A Zhang, Yan %A Fang, Jiasong %T Metabacillus sediminilitoris sp. nov., a marine bacterium isolated from a tidal sediment %D 2020 %J International Journal of Systematic and Evolutionary Microbiology, %V 70 %N 10 %P 5211-5216 %@ 1466-5034 %R https://doi.org/10.1099/ijsem.0.004392 %K tidal sediment %K Metabacillus sediminilitoris %K Bacillus %I Microbiology Society, %X A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17T, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically. The strain could grow at 16–47 °C (optimum 37 °C), at pH 6.0–10.0 (optimum 6.0) and with 1–7% (w/v) NaCl (optimum 3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17T was related to members of the genus Metabacillus and shared the highest similarity with Metabacillus litoralis SW-211T (98.6 %), followed by Metabacillus halosaccharovorans E33T (97.9 %), Metabacillus crassostreae JSM 100118T (97.7 %), Metabacillus niabensis 4T19T (97.7 %) and Metabacillus malikii NCCP-662T (97.5 %). 16S rRNA gene sequence similarities between strain DSL-17T and other members of the genus Metabacillus were below 96.6 %. The sole respiratory quinone was MK-7. Strain DSL-17T had a cell-wall peptidoglycan based on meso-diaminopimelic acid. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), three unidentified glycolipids and six unidentified lipids. The strain had iso-C15 : 0, anteiso-C15 : 0, iso-C14 : 0, C16 : 0 and iso-C16 : 0 as major fatty acids. The G+C content of the genomic DNA was 35.7 mol%. The combined genotypic and phenotypic data indicated that strain DSL-17T represents a novel species of the genus Metabacillus , for which the name Metabacillus sediminilitoris sp. nov. is proposed The type strain is DSL-17T (=MCCC 1K03777T=DSM 109843T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004392