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Volume 70, Issue 10

sp. nov., a marine bacterium isolated from a tidal sediment Free

Abstract

A Gram-stain-positive, endospore-forming, motile and rod-shaped bacterium, designated as strain DSL-17, was isolated from a tidal sediment of the East China Sea and characterized phylogenetically and phenotypically. The strain could grow at 16–47 °C (optimum 37 °C), at pH 6.0–10.0 (optimum 6.0) and with 1–7% (w/v) NaCl (optimum 3 %). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DSL-17 was related to members of the genus and shared the highest similarity with SW-211 (98.6 %), followed by E33 (97.9 %), JSM 100118 (97.7 %), 4T19 (97.7 %) and NCCP-662 (97.5 %). 16S rRNA gene sequence similarities between strain DSL-17 and other members of the genus were below 96.6 %. The sole respiratory quinone was MK-7. Strain DSL-17 had a cell-wall peptidoglycan based on -diaminopimelic acid. The polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), three unidentified glycolipids and six unidentified lipids. The strain had iso-C, anteiso-C, iso-C, C and iso-C as major fatty acids. The G+C content of the genomic DNA was 35.7 mol%. The combined genotypic and phenotypic data indicated that strain DSL-17 represents a novel species of the genus , for which the name sp. nov. is proposed The type strain is DSL-17 (=MCCC 1K03777=DSM 109843).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Bacillus , Metabacillus sediminilitoris and tidal sediment
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41673085)
    • Principle Award Recipient: Yuli Wei
© 2020 The Authors
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2020-08-20
2024-04-23
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Metabacillus sediminilitoris sp. nov., a marine bacterium isolated from a tidal sediment
Int J Syst Evol Microbiol 70, 5211 (2020); https://doi.org/10.1099/ijsem.0.004392
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