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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 9

sp. nov., a new actinobacterium isolated from Moq. rhizosphere Free

Abstract

A novel actinomycete, designated WYY166, was isolated from the rhizosphere of Moq. collected in Dongfang, PR China. The taxonomic position of this strain was investigated using a polyphasic approach. Phylogenetic analysis based on its 16S rRNA gene referred strain WYY166 to the genus , and it was most closely related to the type strains HMC10, DSM 43926, NBRC 106687 and DSM 43925 (98.35, 97.60, 97.36 and 97.30% sequence similarity, respectively). Genome sequencing revealed a genome size of 11.27 Mbp and a G+C content of 71.10 mol%. The genome average nucleotide identity (ANI) values and the digital DNA - DNA hybridization (dDDH) values between strain WYY166 and the other species of the genus were found to be low (ANI 81.63~85.23 %, dDDH 23.6~31.6 %), suggesting that it represented a new species. The physiological evaluation showed that it had remarkable nitrate reduction activity. The whole-cell hydrolysates contained -diaminopimelic acid and madurose. The -acyl type of muramic acid was acetyl. The major menaquinones were MK-9 (H) (86.9 %) and MK-9 (H) (13.1 %). The predominant fatty acids were iso-C (53.2 %), 10-methyl C (10.7 %), C 6 (8.3 %) and iso-C h (7.3 %). These physiological, biochemical and chemotaxonomic data suggested that strain WYY166 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is WYY166 (=MCCC 1K03779=KCTC 49343).

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  • Accepted:
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Keyword(s): actinobacteria , nitrate reduction , Nonomuraea nitratireducens sp. nov. , polyphasic taxonomy and rhizosphere
Funding
This study was supported by the:
  • Fujian Provincial Department of Science and Technology, http://dx.doi.org/10.13039/501100005270 (Award 2019N0018)
  • STCSM International Science and Technology Cooperation Program (Award 18390741000)
  • Startup Fund for Youngman Research at SJTU (Award 17X100040064)
  • Shanghai Municipal Council of Science and Technology (Award 19ZR1475600)
  • National Natural Science Foundation of China (Award 31700027)
  • National Natural Science Foundation of China (Award 21661140002)
  • National Natural Science Foundation of China, http://dx.doi.org/10.13039/501100001809 (Award 31770034)
  • National Key Research and Development Program of China (Award 2018YFA0901900)
© 2020 The Authors
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2024-04-25
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Nonomuraea nitratireducens sp. nov., a new actinobacterium isolated from Suaeda australis Moq. rhizosphere
Int J Syst Evol Microbiol 70, 5026 (2020); https://doi.org/10.1099/ijsem.0.004377
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