@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004374, author = "Gao, Jun-lian and Wang, Li-wei and Xue, Jing and Tong, Shuai and Peng, Guixiang and Sun, Yu-chen and Zhang, Xiuhai and Sun, Jian-guang", title = "Rhizobium rhizophilum sp. nov., an indole acetic acid-producing bacterium isolated from rape (Brassica napus L.) rhizosphere soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "9", pages = "5019-5025", doi = "https://doi.org/10.1099/ijsem.0.004374", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004374", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "indole acetic acid producing bacterium", keywords = "polyphasic taxonomy", keywords = "Rhizobium rhizophilum", keywords = "rape (Brassica napus L.)", abstract = "A novel Gram-stain-negative, aerobic, rod-shaped and indole acetic acid-producing strain, designated 7209-2T, was isolated from rhizosphere of rape (Brassica napus L.) grown in the Yakeshi City, Inner Mongolia, PR China. The 16S rRNA gene sequence analysis indicated that strain 7209-2T belongs to the genus Rhizobium and is closely related to Rhizobium rosettiformans W3T, Rhizobium ipomoeae shin9-1T and Rhizobium wuzhouense W44T with sequence similarities of 98.2, 98.1 and 97.9 %, respectively. Phylogenetic analysis based on concatenated housekeeping recA and atpD gene sequences showed that strain 7209-2T formed a group together with R. wuzhouense W44T and R. rosettiformans W3T, with sequences similarities of 92.6 and 91.1 %, respectively. The genome size of strain 7209-2T was 5.25 Mb, comprising 5027 predicted genes with a DNA G+C content of 61.2 mol%. The average nucleotide identity and digital DNA–DNA hybridization comparisons among 7209-2T and reference strains for the most closely related species showed values below the accepted threshold for species discrimination. The major fatty acids of strain 7209-2T were summed feature 8 (C18 : 1  ω7c and/or C18 : 1  ω6c) and summed feature 2 (C12 : 0 aldehyde and/or unknown 10.953) . The major polar lipids were found to consist of phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and an unidentified aminophospholipid. The predominant ubiquinone was identified as quinone 10. Based on all the above results, strain 7209-2T represents a novel species of the genus Rhizobium , for which the name Rhizobium rhizophilum sp. nov. is proposed with 7209-2T (=CGMCC 1.15691T=DSM 103161T) as the type strain.", }