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Abstract

A halophilic archaeon named strain LR21 was isolated from a salt mine in Yunnan Province, PR China. Cells were spherical, Gram-stain-negative and motile. Strain LR21 grew at 20–50 °C (optimum, 42 °C), with 8–30 % (w/v) NaCl (optimum, 23 %) and at pH 5.5–9.5 (optimum, pH 7.5–8.5). Mg was not required for growth. The major polar lipid profile comprised phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester and phosphatidylglycerol sulfate. Strain LR21 had two dissimilar 16S rRNA genes ( and ) and they were closely related to YGHS32, BNERC31 and YGHS18 with sequence similarities of 95.3–99.0, 93.0–96.2 and 93.2–95.9 %, respectively, and much lower values to other members. The ′ gene sequence similarities between strain LR21 and YGHS32, BNERC31 and YGHS18 were 95.2, 91.2 and 91.2 % respectively. The values of average nucleotide identity (ANI) and average amino-acid identity (AAI) between strain LR21 and YGHS32, were 89.0 and 90.1 %, respectively. DNA relatedness between strains LR21 and YGHS32 determined by DNA–DNA hybridization was 36.8 %. Values of ANI and AAI between strain LR21 and other members in the genus were far below 95 % and the DNA–DNA relatedness values between strain LR21 and its close relatives were much lower than 70 %, which is far below the boundary for delineation of a new species prokaryote. The DNA G+C content of strain LR21 was 62.0 mol% (genome). The results suggested that strain LR21 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is LR21 (=CGMCC 1.13611=NBRC 113588).

Funding
This study was supported by the:
  • Anhui Provincial Natural Science Research Project (Award KJ2019A0504)
    • Principle Award Recipient: Shaoxing Chen
  • Excellent Young Talents Fund Project for Universities in Anhui Province, China (Award gxyqZD2017011)
    • Principle Award Recipient: Shaoxing Chen
  • the Anhui Provincial Key Lab. of the Conservation and Exploitation of Biological Resources (Award 591601)
    • Principle Award Recipient: Shaoxing Chen
  • National Natural Science Foundation of China (Award 31460003)
    • Principle Award Recipient: Shaoxing Chen
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2020-08-12
2021-08-04
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