@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004365, author = "Li, Jianyang and Qi, Mingming and Lai, Qiliang and Liu, Xianhua and Shao, Zongze", title = "Ketobacter nezhaii sp. nov., a marine bacterium isolated from coastal sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "9", pages = "4960-4965", doi = "https://doi.org/10.1099/ijsem.0.004365", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004365", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "taxonomy", keywords = "Ketobacter", abstract = "A Gram-stain-negative, motile, aerobic and heterotrophic bacterium, designated as GYS_M3HT, was isolated from marine coastal sediment sampled at Xiamen Island. Cells were rod-shaped with one polar flagellum and weakly positive for oxidase and catalase. Growth of the strain occurred at pH 6–9 (optimum, pH 7–8), at 15–37 °C (optimum, 28 °C) and with NaCl concentrations of 1.0–6.0 % (optimum, 2.0 %). It had highest 16S rRNA similarity (97.7 %) to Ketobacter alkanivorans GI5T, followed by the members of the genus Alcanivorax (lower than 91.2 %). The results of phylogenetic analysis indicated that it belonged to the genus Ketobacter within the family Alcanivoracaceae . In addition, the average nucleotide identity and digital DNA–DNA hybridization values between strain GYS_M3HT and K. alkanivorans GI5T were 71.4 and 19.7 %, respectively, indicating that strain GYS_M3HT belonged to a novel species. Its genome consisted of 5 318 758 bp, with a genomic DNA G+C content of 50.0 mol%. The respiratory quinone was Q-8 and the dominant fatty acids were identified as iso-C15 : 0 (25.4 %), C16 : 1  ω6c/C16 : 1  ω7c (14.4 %) and iso-C13 : 0 (7.2 %). The main polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Therefore, based on phenotypic, chemotaxonomic and phylogenetic results, strain GYS_M3HT represents a novel species within the genus Ketobacter , for which the name Ketobacter nezhaii sp. nov. is proposed, with the type strain GYS_M3HT (=MCCC 1A13808T=KCTC 72247T).", }