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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 70, Issue 9

sp. nov. a marine bacterium isolated from coastal sediment Free

Abstract

A Gram-stain-negative, motile, aerobic and heterotrophic bacterium, designated as GYS_M3H, was isolated from marine coastal sediment sampled at Xiamen Island. Cells were rod-shaped with one polar flagellum and weakly positive for oxidase and catalase. Growth of the strain occurred at pH 6–9 (optimum, pH 7–8), at 15–37 °C (optimum, 28 °C) and with NaCl concentrations of 1.0–6.0 % (optimum, 2.0 %). It had highest 16S rRNA similarity (97.7 %) to GI5, followed by the members of the genus (lower than 91.2 %). The results of phylogenetic analysis indicated that it belonged to the genus within the family . In addition, the average nucleotide identity and digital DNA–DNA hybridization values between strain GYS_M3H and GI5 were 71.4 and 19.7 %, respectively, indicating that strain GYS_M3H belonged to a novel species. Its genome consisted of 5 318 758 bp, with a genomic DNA G+C content of 50.0 mol%. The respiratory quinone was Q-8 and the dominant fatty acids were identified as iso-C (25.4 %), C ω6c/C ω7 (14.4 %) and iso-C (7.2 %). The main polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Therefore, based on phenotypic, chemotaxonomic and phylogenetic results, strain GYS_M3H represents a novel species within the genus , for which the name sp. nov. is proposed, with the type strain GYS_M3H (=MCCC 1A13808=KCTC 72247).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Ketobacter and taxonomy
Funding
This study was supported by the:
  • National Infrastructure of Microbial Resources of China (Award NIMR-2020-9)
    • Principle Award Recipient: Zongze Shao
  • COMRA program (Award DY135-B2-01)
    • Principle Award Recipient: Zongze Shao
  • NSFC Hydrosphere Project (Award 91851203)
    • Principle Award Recipient: Zongze Shao
© 2020 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.004365
2020-08-07
2025-01-22
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Ketobacter nezhaii sp. nov., a marine bacterium isolated from coastal sediment
Int J Syst Evol Microbiol 70, 4960 (2020); https://doi.org/10.1099/ijsem.0.004365
/content/journal/ijsem/10.1099/ijsem.0.004365
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