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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 9

gen. nov., sp. nov., a novel genus of the family , isolated from the gut of plastic-eating larvae of the Coleoptera insect Free

Abstract

A bacterial strain, BIT-B35, was isolated from the gut of plastic-eating larvae of the Coleoptera insect . Its taxonomic position was determined by using a polyphasic approach. Cells were white-pigmented, Gram-stain-negative, motile short rods with terminal flagella. The 16S rRNA gene sequence (1411 bp) of strain BIT-B35 showed highest similarity (98.1%) to ATCC 35469 and LMG 5519. The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of seven housekeeping genes (, , , , , and ) and genome sequences, placed strain BIT-B35 in a separate lineage among the family of . The major fatty acids were C, C cyclo and C cyclo 8. The genomic DNA G+C content of strain BIT-B35 was 57.1 mol%. The chemotaxonomic data plus results of physiological and biochemical tests also distinguished strain BIT-B35 from members of other genera within the family . Therefore, strain BIT-B35 is considered to represent a novel species of a novel genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is BIT-B35 (=CGMCC 1.17042=KCTC 72448).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): gut bacteria , Intestinirhabdus , plastic biodegradation , polystyrene and Zophobas
Funding
This study was supported by the:
  • Young Elite Scientist Sponsorship Program of the China Association of Science and Technology (Award 2017QNRC001)
    • Principle Award Recipient: Yu Yang
  • National Natural Science Foundation of China (Award 51603004)
    • Principle Award Recipient: Yu Yang
  • National Natural Science Foundation of China (Award 31961133015)
    • Principle Award Recipient: Yu Yang
© 2020 The Authors
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2020-08-03
2024-04-19
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Intestinirhabdus alba gen. nov., sp. nov., a novel genus of the family Enterobacteriaceae, isolated from the gut of plastic-eating larvae of the Coleoptera insect Zophobas atratus
Int J Syst Evol Microbiol 70, 4951 (2020); https://doi.org/10.1099/ijsem.0.004364
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