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Abstract

A novel bacterial strain, designated TBM-1, isolated from a freshwater lake in Taiwan, was characterized using a polyphasic taxonomic approach. Phylogenetic analyses based on 16S rRNA gene sequences and coding sequences of 92 protein clusters indicated that strain TBM-1 formed a phylogenetic lineage in the genus . Analysis of 16S rRNA gene sequences showed that strain TBM-1 was most closely related to CCUG 30898 with 98.4 % sequence similarity. The average nucleotide identity, average amino acid identity and digital DNA–DNA hybridization values between strain TBM-1 and closely related strains of the genus were 74.4–77.5 %, 69.7–75.4 % and 19.8–21.8 %, respectively, supporting that strain TBM-1 represents a novel species of the genus . Cells were Gram-stain-negative, motile by means of a single polar flagellum, rod-shaped and formed blue colonies. Optimal growth occurred at 30 °C, pH 6 and 0 % NaCl. The predominant fatty acids of strain TBM-1 were summed feature 3 (C ω7 and/or C ω6), C ω7 and C. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, two uncharacterized aminophospholipids and two uncharacterized phospholipids. The main polyamine was putrescine. The major isoprenoid quinone was Q-8. The estimated genome size was 5.26 Mb, with an average G+C content of 70.0 mol%. On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TBM-1 should be classified in a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TBM-1 (=BCRC 81199 =LMG 31339).

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2020-08-04
2021-07-29
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