RT Journal Article SR Electronic(1) A1 Muramatsu, So A1 Kanamuro, Masataka A1 Sato-Takabe, Yuki A1 Hirose, Setsuko A1 Muramatsu, Yuki A1 Takaichi, Shinichi A1 Hanada, SatoshiYR 2020 T1 Roseobacter cerasinus sp. nov., isolated from a fish farm JF International Journal of Systematic and Evolutionary Microbiology, VO 70 IS 9 SP 4920 OP 4926 DO https://doi.org/10.1099/ijsem.0.004360 PB Microbiology Society, SN 1466-5034, AB An obligate aerobic and bacteriochlorophyll a-containing bacterium, designated strain AI77T, was isolated from a fish farm in Uwa Sea, Japan. Cells were Gram-stain-negative, coccoid- to oval-shaped, and showed no motility. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain AI77T is a member of the genus Roseobacter and closely related to Roseobacter ponti MM-7T (97.8 %), Roseobacter denitrificans OCh 114T (97.3 %) and Roseobacter litoralis OCh 149T (97.3 %). The G+C content of strain AI77T was 61.0 mol%. The average amino acid identity values of the genome in strain AI77T with those in R. denitrificans OCh 114T and R. litoralis OCh 149T were 73.26 % (SD 16.46) and 72.63 % (SD 16.76), respectively. The digital DNA–DNA hybridization values of strain AI77T with the type strains R. denitrificans OCh 114T and R. litoralis OCh 149T were 18.70 and 18.50 %, respectively. The dominant fatty acids (>10 % of total fatty acids) of AI77T were summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and saturated fatty acid C16 : 0. The sole respiratory quinone was ubiquinone-10. The predominant polar lipids were phosphatidylcholine, phosphatidylglycerol and diphosphatidylglycerol. Based on the genetic and phenotypic data obtained herein, we conclude that strain AI77T represents a new species of the genus Roseobacter , for which we propose the name Roseobacter cerasinus sp. nov.; the type strain is AI77T (=DSM 110091T=NBRC 114115T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004360