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Volume 70, Issue 8

sp. nov., sp. nov., sp. nov. and sp. nov., isolated from a subtropical stream in PR China and proposal to reclassify as comb. nov. Free

Abstract

Five Gram-stain-negative, catalase- and oxidase-positive, rod-shaped and motile strains (FT50W, FT80W, FT92W, FT94W and FT135W) were isolated from a subtropical stream in PR China. Comparisons based on 16S rRNA gene sequences showed that strains FT50W, FT94W and FT135W take strain Sac-22, and strains FT80W and FT92W take strain DCY83 as their closest neighbour in the phylogenetic trees, respectively. The G+C contents of strains FT50W, FT80W, FT92W, FT94W and FT135W were 63.3, 62.4, 62.8, 63.8 and 60.8 %, respectively. The reconstructed phylogenomic tree based on concatenated 92 core genes showed that strains FT50W, FT80W, FT94W and FT135W clustered together with species of the genus , but strains FT92W and KCTC 42409 were located in the clades of the genus . The calculated pairwise average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values among strains FT50W, FT80W, FT92W, FT94W, FT135W and related strains were in the ranges of 75.6–87.8% and 20.3–33.8% except that the values between strains FT50W and FT94W were 98.7 and 89.2%, respectively. The respiratory quinone of these five strains was Q-8. The major fatty acids were C 7, C, C ω7 and C. The polar lipids included phosphatidylethanolamine, phosphatidylglycerol and one unidentified phospholipid. Considering the distinct phylogenetic relationships of with species of the genus in the phylogenomic tree, it was reasonable to transfer to the genus as comb. nov. Combining the results of phylogenomic analysis, ANI and dDDH data, and a range of physiological and biochemical characteristics together, strains FT50W and FT94W should belong to the same species and be assigned to genus with strains FT80W and FT135W together, and strain FT92W should be assigned to the genus , for which the names sp. nov. (type strain FT50W=GDMCC 1.1674=KACC 21466), sp. nov. (FT80W=GDMCC 1.1678=KACC 21470), sp. nov. (FT135W=GDMCC 1.1745=KACC 21659) and sp. nov. (FT92W=GDMCC 1.1682=KACC 21474) are proposed.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Duganella , Massilia , polyphasic taxonomy and subtropical stream
Funding
This study was supported by the:
  • Key-Area Research and Development Program of Guangdong Province, China (Award No. 2018B020205003)
    • Principle Award Recipient: Meiying Xu
  • Guangdong MEPP Fund (Award NO. GDOE(2019)A34)
    • Principle Award Recipient: Meiying Xu
  • GDAS’ Special Project of Science and Technology Development (Award 2019GDASYL-0301002; 2020GDASYL-20200402001)
    • Principle Award Recipient: Meiying Xu
  • Guangdong Provincial Programs for Science and Technology Development (Award 2019B110205004; 2018B030324002)
    • Principle Award Recipient: Meiying Xu
  • National Natural Science Foundation of China (Award 91851202, 51678163)
    • Principle Award Recipient: Meiying Xu
© 2020 The Authors
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2024-04-25
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Duganella lactea sp. nov., Duganella guangzhouensis sp. nov., Duganella flavida sp. nov. and Massilia rivuli sp. nov., isolated from a subtropical stream in PR China and proposal to reclassify Duganella ginsengisoli as Massilia ginsengisoli comb. nov.
Int J Syst Evol Microbiol 70, 4822 (2020); https://doi.org/10.1099/ijsem.0.004355
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