@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004354, author = "Hu, Yuzhong and Guo, Yu and Lai, Qiliang and Dong, Le and Huang, Zhaobin", title = "Draconibacterium mangrovi sp. nov., isolated from mangrove sediment", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "8", pages = "4816-4821", doi = "https://doi.org/10.1099/ijsem.0.004354", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004354", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "mangrove sediment", keywords = "Draconibacterium mangrovi", keywords = "polyphasic taxonomy", abstract = "A marine bacterial strain, designated GM2-18T, was isolated from mangrove sediment sampled at Luoyang River estuary, Quanzhou, PR China. Cells were Gram-stain-negative, slightly curved long rod-shaped and facultatively anaerobic with no flagellum. Catalase activity was found to be weak-positive and oxidase-positive. It had no ability to degrade or hydrolyse substrates including skimmed milk, cellulose, starch and Tweens (40, 60 and 80). The 16S rRNA gene sequence of strain GM2-18T had maximum similarity values to ‘ Draconibacterium filum ’ F2T, Draconibacterium sediminis JN14CK-3T and Draconibacterium orientale FH5T of 98.0, 97.8 and 97.4 %, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GM2-18T was affiliated to the genus Draconibacterium and formed a clade with an uncultured bacterium clone identified from mangrove environment. Average nucleotide identity values and DNA–DNA hybridization estimates of strain GM2-18T compared to its Draconibacterium relatives strongly supported that it belonged to a new species. The respiratory quinone was menaquinone MK-7. The major fatty acids (>10 %) consisted of iso-C15 : 0, anteiso-C15 : 0 and C17 : 1  ω6c. The polar lipids were phosphatidylethanolamine, a phospholipid and several unidentified lipids. The genomic size of strain GM2-18T was 5.9 Mb and the G+C content was 40.8 mol%. Gene prediction and annotation of strain GM2-18T indicated that there was a nitrogen-fixing gene cluster encoding nitrogenase molybdenum-iron protein and related proteins responsible for nitrogen fixation. Based on the above characteristics, strain GM2-18T represents a novel species within the genus Draconibacterium . Thus, Draconibacterium mangrovi sp. nov. is proposed with type strain GM2-18T (=MCCC 1K04382T=KCTC 72879T), isolated from mangrove sediment.", }