1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 8

sp. nov., isolated from mangrove sediment Free

Abstract

A marine bacterial strain, designated GM2-18, was isolated from mangrove sediment sampled at Luoyang River estuary, Quanzhou, PR China. Cells were Gram-stain-negative, slightly curved long rod-shaped and facultatively anaerobic with no flagellum. Catalase activity was found to be weak-positive and oxidase-positive. It had no ability to degrade or hydrolyse substrates including skimmed milk, cellulose, starch and Tweens (40, 60 and 80). The 16S rRNA gene sequence of strain GM2-18 had maximum similarity values to ‘’ F2, JN14CK-3 and FH5 of 98.0, 97.8 and 97.4 %, respectively. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain GM2-18 was affiliated to the genus and formed a clade with an uncultured bacterium clone identified from mangrove environment. Average nucleotide identity values and DNA–DNA hybridization estimates of strain GM2-18 compared to its relatives strongly supported that it belonged to a new species. The respiratory quinone was menaquinone MK-7. The major fatty acids (>10 %) consisted of iso-C, anteiso-C and C 6. The polar lipids were phosphatidylethanolamine, a phospholipid and several unidentified lipids. The genomic size of strain GM2-18 was 5.9 Mb and the G+C content was 40.8 mol%. Gene prediction and annotation of strain GM2-18 indicated that there was a nitrogen-fixing gene cluster encoding nitrogenase molybdenum-iron protein and related proteins responsible for nitrogen fixation. Based on the above characteristics, strain GM2-18 represents a novel species within the genus . Thus, sp. nov. is proposed with type strain GM2-18 (=MCCC 1K04382=KCTC 72879), isolated from mangrove sediment.

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Keyword(s): Draconibacterium mangrovi , mangrove sediment and polyphasic taxonomy
Funding
This study was supported by the:
  • marine microbial collection program as part of the fund of National Microbial Resource Center (No. NMRC-2020-9) (Award 2019KJ25)
    • Principle Award Recipient: Zhaobin Huang
© 2020 The Authors
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2020-07-24
2024-04-25
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Draconibacterium mangrovi sp. nov., isolated from mangrove sediment
Int J Syst Evol Microbiol 70, 4816 (2020); https://doi.org/10.1099/ijsem.0.004354
/content/journal/ijsem/10.1099/ijsem.0.004354
/content/journal/ijsem/10.1099/ijsem.0.004354
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