@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004353, author = "Liu, Zi-Xuan and Phurbu, Dorji and Liu, Hong-Can and Zhou, Yu-Guang and Li, Ai-Hua", title = "Craterilacuibacter sinensis gen. nov. sp. nov., isolated from a crater lake in China", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "8", pages = "4831-4837", doi = "https://doi.org/10.1099/ijsem.0.004353", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004353", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Neisseriaceae", keywords = "Craterilacuibacter", keywords = "Buteha crater lake", abstract = "Two bacterial strains, designated B2N2-7T and B2N2-12, were isolated from Buteha crater lake in the Greater Khingan Mountain of China. The two strains were Gram-stain-negative, non-spore-forming, motile with a single polar flagellum, short rod-shaped bacteria. They were catalase- and oxidase-positive. Optimal growth occurred at 20–25 ℃, at pH 7.5–8.0 and with 0–1.0 % (w/v) NaCl. Based on phylogenomic analysis, strains B2N2-7T and B2N2-12 were assigned to the family Neisseriaceae , and their 16S rRNA gene sequences showed the highest similarities to that of Aquitalea denitrificans 5YN1-3T (<94.2 %). The predominant cellular fatty acids were C16 : 0 and summed feature 3 (comprising C16 : 1ω7c/C16 : 1 ω6c). The major respiratory quinone was ubiquinone 8 (Q-8). The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL) and some unidentified lipids (L). The genomic DNA G+C content of strain B2N2-7T was 59.4 mol% according to the genomic sequencing result. Based on the phylogenetic, genotypic and chemotaxonomic analyses, the two strains are proposed to represent a novel species of a new genus in the family Neisseriaceae , named Craterilacuibacter sinensis gen. nov., sp. nov. The type strain of Craterilacuibacter sinensis is B2N2-7T (=CGMCC 1.17189T=KCTC 73735T); B2N2-12 (=CGMCC 1.17190=KCTC 72734) is a second strain of the species.", }