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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 8

gen. nov. sp. nov., isolated from a crater lake in China Free

Abstract

Two bacterial strains, designated B2N2-7 and B2N2-12, were isolated from Buteha crater lake in the Greater Khingan Mountain of China. The two strains were Gram-stain-negative, non-spore-forming, motile with a single polar flagellum, short rod-shaped bacteria. They were catalase- and oxidase-positive. Optimal growth occurred at 20–25 ℃, at pH 7.5–8.0 and with 0–1.0 % (w/v) NaCl. Based on phylogenomic analysis, strains B2N2-7 and B2N2-12 were assigned to the family , and their 16S rRNA gene sequences showed the highest similarities to that of 5YN1-3 (<94.2 %). The predominant cellular fatty acids were C and summed feature 3 (comprising Cω/C 6). The major respiratory quinone was ubiquinone 8 (Q-8). The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL) and some unidentified lipids (L). The genomic DNA G+C content of strain B2N2-7 was 59.4 mol% according to the genomic sequencing result. Based on the phylogenetic, genotypic and chemotaxonomic analyses, the two strains are proposed to represent a novel species of a new genus in the family , named gen. nov., sp. nov. The type strain of is B2N2-7 (=CGMCC 1.17189=KCTC 73735); B2N2-12 (=CGMCC 1.17190=KCTC 72734) is a second strain of the species.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Buteha crater lake , Craterilacuibacter and Neisseriaceae
Funding
This study was supported by the:
  • the National Natural Science Funds of China (Award 31970004)
    • Principle Award Recipient: Ai-Hua Li
© 2020 China General Microbiological Culture Collection Center
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2020-07-23
2024-04-19
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References

  1. Prévot AR. Etude de systématique bactérienne. I. Lois générales. II. cocci anaérobies.. Annales des Sciences Naturelles Zoologie et Biologie Animale 1933; 15:23–260
     [Google Scholar]
  2. Trevisan V. Carratteri di alcuni nuovi generi di Batteriacee. Atti Accad Fis Med Stat Milano Ser 1885; 4:92–107
     [Google Scholar]
  3. Moß KS, Hartmann SC, Müller I, Fritz C, Krügener S et al. Amantichitinum ursilacus gen. nov., sp. nov., a chitin-degrading bacterium isolated from soil. Int J Syst Evol Microbiol 2013; 63:98–103 [View Article]
     [Google Scholar]
  4. Sato K, Kato Y, Taguchi G, Nogawa M, Yokota A et al. Chitiniphilus shinanonensis gen. nov., sp. nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria. J Gen Appl Microbiol 2009; 55:147–153 [View Article]
     [Google Scholar]
  5. Chen W-M, Yang S-H, Huang W-C, Cheng C-Y, Sheu S-Y. Chitinivorax tropicus gen. nov., sp. nov., a chitinolytic bacterium isolated from a freshwater lake. Int J Syst Evol Microbiol 2012; 62:1086–1091 [View Article]
     [Google Scholar]
  6. Dong L, Ming H, Zhou E-M, Yin Y-R, Liu L et al. Crenobacter luteus gen. nov., sp. nov., isolated from a hot spring. Int J Syst Evol Microbiol 2015; 65:214–219 [View Article]
     [Google Scholar]
  7. Yoon J-H, Choi JH, Kang S-J, Choi N-S, Lee J-S et al. Jeongeupia naejangsanensis gen. nov., sp. nov., a cellulose-degrading bacterium isolated from forest soil from Naejang Mountain in Korea. Int J Syst Evol Microbiol 2010; 60:615–619 [View Article]
     [Google Scholar]
  8. Sheu S-Y, Chen J-C, Young C-C, Chen W-M. Rivicola pingtungensis gen. nov., sp. nov., a new member of the family Neisseriaceae isolated from a freshwater river. Int J Syst Evol Microbiol 2014; 64:2009–2016 [View Article]
     [Google Scholar]
  9. Kwong WK, Moran NA. Cultivation and characterization of the gut symbionts of honey bees and bumble bees: description of Snodgrassella alvi gen. nov., sp. nov., a member of the family Neisseriaceae of the Betaproteobacteria and Gilliamella apicola gen. nov., sp. nov., a member of Orbaceae fam. nov., Orbales ord. nov., a sister taxon to the order ‘Enterobacteriales’ of the Gammaproteobacteria . Int J Syst Evol Microbiol 2013; 63:2008–2018 [View Article]
     [Google Scholar]
  10. Karlson U, Dwyer DF, Hooper SW, Moore ER, Timmis KN et al. Two independently regulated cytochromes P-450 in a Rhodococcus rhodochrous strain that degrades 2-ethoxyphenol and 4-methoxybenzoate. J Bacteriol 1993; 175:1467–1474 [View Article]
     [Google Scholar]
  11. Lane DJ, Goodfellow M. 16S/23S rRNA sequencing. In Stackebrandt E. editor Nucleic Acid Techniques in Bacterial Systematics Chichester:Wiley: 1991 pp 115–175
     [Google Scholar]
  12. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [View Article]
     [Google Scholar]
  13. Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article]
     [Google Scholar]
  14. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [View Article]
     [Google Scholar]
  15. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425
     [Google Scholar]
  16. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
     [Google Scholar]
  17. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 1971; 20:406–416 [View Article]
     [Google Scholar]
  18. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article]
     [Google Scholar]
  19. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
     [Google Scholar]
  20. Lee C-M, Weon H-Y, Kim Y-J, Son J-A, Yoon S-H et al. Aquitalea denitrificans sp. nov., isolated from a Korean wetland. Int J Syst Evol Microbiol 2009; 59:1045–1048 [View Article]
     [Google Scholar]
  21. Grimes DJ, Woese CR, MacDonell MT, Colwell RR. Systematic study of the genus Vogesella gen. nov. and its type species, Vogesella indigofera comb. nov. Int J Syst Bacteriol 1997; 47:19–27 [View Article]
     [Google Scholar]
  22. Parks DH, Imelfort M, Skennerton CT, Hugenholtz P, Tyson GW et al. CheckM: assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. Genome Res 2015; 25:1043–1055 [View Article]
     [Google Scholar]
  23. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 2018; 68:461–466 [View Article]
     [Google Scholar]
  24. Davis JJ, Wattam AR, Aziz RK, Brettin T, Butler R et al. The PATRIC bioinformatics resource center: expanding data and analysis capabilities. Nucleic Acids Res 2020; 48:D606–D612
     [Google Scholar]
  25. Pheng S, Lee JJ, Eom MK, Lee KH, Kim S-G et al. Paucibacter oligotrophus sp. nov., isolated from fresh water, and emended description of the genus Paucibacter . Int J Syst Evol Microbiol 2017; 67:2231–2235 [View Article]
     [Google Scholar]
  26. Gerhardt P, Murray RGE, Wood WA, Krieg NR. Methods for General and Molecular Bacteriology Washington, DC: Amer Soc Microbiol; 1994
     [Google Scholar]
  27. Miller LT. Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 1982; 16:584–586 [View Article]
     [Google Scholar]
  28. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. NewYork: MIDI Inc; 1990
     [Google Scholar]
  29. Collins MD. Isoprenoid quinone analysis in classification and identification. In Goodfellow M, Minnikin DE. (editors) Chemical Methods in Bacterial Systematics London: Academic Press; 1985 pp 267–287
     [Google Scholar]
  30. Wu C, Lu X, Qin M et al. Analysis of menaquinone compound in microbial cells by HPLC. Microbiology [English translation of Microbiology (Beijing)] 1989; 16:176–178
     [Google Scholar]
  31. Minnikin DE, Bolton RC, Hartmann S et al. An integrated procedure for the direct detection of characteristic lipids in tuberculosis patients. Ann Soc Belg Med Trop 1993; 73:13–24
     [Google Scholar]
  32. Subhash Y, Tushar L, Sasikala C, Ramana CV, Ch R V. Vogesella alkaliphila sp. nov., isolated from an alkaline soil, and emended description of the genus Vogesella . Int J Syst Evol Microbiol 2013; 63:2338–2343 [View Article]
     [Google Scholar]
  33. Lin M-C, Chou J-H, Arun AB, Young C-C, Chen W-M. Pseudogulbenkiania subflava gen. nov., sp. nov., isolated from a cold spring. Int J Syst Evol Microbiol 2008; 58:2384–2388 [View Article]
     [Google Scholar]
  34. Vaz-Moreira I, Nobre MF, Nunes OC, Manaia CM. Gulbenkiania mobilis gen. nov., sp. nov., isolated from treated municipal wastewater. Int J Syst Evol Microbiol 2007; 57:1108–1112 [View Article]
     [Google Scholar]
  35. Kwon S-W, Kim B-Y, Kim W-G, Yoo K-H, Yoo S-H et al. Paludibacterium yongneupense gen. nov., sp. nov., isolated from a wetland, Yongneup, in Korea. Int J Syst Evol Microbiol 2008; 58:190–194 [View Article]
     [Google Scholar]
  36. Sedláček I, Kwon S-W et al. Aquitalea pelogenes sp. nov., isolated from mineral peloid. Int J Syst Evol Microbiol 2016; 66:962–967 [View Article]
     [Google Scholar]
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Craterilacuibacter sinensis gen. nov. sp. nov., isolated from a crater lake in China
Int J Syst Evol Microbiol 70, 4831 (2020); https://doi.org/10.1099/ijsem.0.004353
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