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Abstract

Two bacterial strains, designated B2N2-7 and B2N2-12, were isolated from Buteha crater lake in the Greater Khingan Mountain of China. The two strains were Gram-stain-negative, non-spore-forming, motile with a single polar flagellum, short rod-shaped bacteria. They were catalase- and oxidase-positive. Optimal growth occurred at 20–25 ℃, at pH 7.5–8.0 and with 0–1.0 % (w/v) NaCl. Based on phylogenomic analysis, strains B2N2-7 and B2N2-12 were assigned to the family , and their 16S rRNA gene sequences showed the highest similarities to that of 5YN1-3 (<94.2 %). The predominant cellular fatty acids were C and summed feature 3 (comprising Cω/C 6). The major respiratory quinone was ubiquinone 8 (Q-8). The polar lipids were phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), two unidentified aminophospholipids (APL) and some unidentified lipids (L). The genomic DNA G+C content of strain B2N2-7 was 59.4 mol% according to the genomic sequencing result. Based on the phylogenetic, genotypic and chemotaxonomic analyses, the two strains are proposed to represent a novel species of a new genus in the family , named gen. nov., sp. nov. The type strain of is B2N2-7 (=CGMCC 1.17189=KCTC 73735); B2N2-12 (=CGMCC 1.17190=KCTC 72734) is a second strain of the species.

Funding
This study was supported by the:
  • Ai-Hua Li , the National Natural Science Funds of China , (Award 31970004)
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2020-07-23
2020-09-20
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