1887

Abstract

A Gram-stain-negative, facultative anaerobic, motile, short rods and yellow-pigmented bacterium, designated strain THG-DN7.12, was isolated from water collected at Jungwon waterfall on Yongmun mountain, Republic of Korea. According to 16S rRNA gene sequence comparisons, strain THG-DN7.12 was found to be most closely related to 5YN1-3 (98.9 % sequence similarity), TRO-001DR8 (98.7 %) and P1297 (98.0 %). The DNA–DNA relatedness between strain THG-DN7.12 and its phylogenetically closest neighbours was below 70.0 %. The strain's DNA G+C content was 59.7 mol%. The major polar lipid was found to be phosphatidylethanolamine. Summed feature 3 (C 7 and/or C 6) and C were identified as the major fatty acids. Ubiquinone Q-8 was detected as the only respiratory quinone. These data supported the affiliation of strain THG-DN7.12 to the genus . Strain THG-DN7.12 was distinguished from related species by physiological and biochemical tests. Therefore, the novel isolate represents a novel species, for which the name sp. nov. is proposed, with THG-DN7.12 as the type strain (=KACC 18847=CCTCC AB 2016185).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004351
2020-08-21
2020-09-28
Loading full text...

Full text loading...

References

  1. Lau H-T, Faryna J, Triplett EW. Aquitalea magnusonii gen. nov., sp. nov., a novel Gram-negative bacterium isolated from a humic lake. Int J Syst Evol Microbiol 2006; 56:867–871 [CrossRef][PubMed]
    [Google Scholar]
  2. Sedláček I, Kwon S-W, Mašlanˇová I, Kýrová K et al. Aquitalea pelogenes sp. nov., isolated from mineral peloid. Int J Syst Evol Microbiol 2016; 66:962–967 [CrossRef][PubMed]
    [Google Scholar]
  3. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [CrossRef][PubMed]
    [Google Scholar]
  4. Kim O-S, Cho Y-J, Lee K, Yoon S-H, Kim M et al. Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. Int J Syst Evol Microbiol 2012; 62:716–721 [CrossRef][PubMed]
    [Google Scholar]
  5. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [CrossRef][PubMed]
    [Google Scholar]
  6. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  7. Kimura M. The Neutral Theory of Molecular Evolution Cambridge University Press; 1983
    [Google Scholar]
  8. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [CrossRef][PubMed]
    [Google Scholar]
  9. Tamura K, Peterson D, Peterson N, Stecher G, Nei M et al. MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 2011; 28:2731–2739 [CrossRef][PubMed]
    [Google Scholar]
  10. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791 [CrossRef][PubMed]
    [Google Scholar]
  11. Ezaki T, Hashimoto Y, Yabuuchi E. Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 1989; 39:224–229 [CrossRef]
    [Google Scholar]
  12. Stabili L, Gravili C, Tredici SM, Piraino S, Talà A et al. Epibiotic Vibrio luminous bacteria isolated from some Hydrozoa and Bryozoa species. Microb Ecol 2008; 56:625–636 [CrossRef][PubMed]
    [Google Scholar]
  13. Lee C-M, Weon H-Y, Kim Y-J, Son J-A, Yoon S-H et al. Aquitalea denitrificans sp. nov., isolated from a Korean wetland. Int J Syst Evol Microbiol 2009; 59:1045–1048 [CrossRef][PubMed]
    [Google Scholar]
  14. Wayne LG, Moore WEC, Stackebrandt E, Kandler O, Colwell RR, Brenner DJ, Grimont PA et al. Report of the ad hoc Committee on reconciliation of approaches to bacterial Systematics. Int J Syst Evol Microbiol 1987; 37:463–464 [CrossRef]
    [Google Scholar]
  15. Stackebrandt E, Goebel BM. Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Evol Microbiol 1994; 44:846–849 [CrossRef]
    [Google Scholar]
  16. Yoon S-H, Ha S-M, Kwon S, Lim J, Kim Y et al. Introducing EzBioCloud: a taxonomically United database of 16S rRNA gene sequences and whole-genome assemblies. Int J Syst Evol Microbiol 2017; 67:1613–1617 [CrossRef][PubMed]
    [Google Scholar]
  17. Kim M, Oh H-S, Park S-C, Chun J. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol 2014; 64:346–351 [CrossRef][PubMed]
    [Google Scholar]
  18. Minnikin DE, O'Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [CrossRef]
    [Google Scholar]
  19. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI Inc; 1990
    [Google Scholar]
  20. Collins MD, Jones D. Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. Microbiol Rev 1981; 45:316–354 [CrossRef][PubMed]
    [Google Scholar]
  21. Tamaoka J, Katayama-Fujimura Y, Kuraishi H. Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. J Appl Bacteriol 1983; 54:31–36 [CrossRef]
    [Google Scholar]
  22. Hiraishi A, Ueda Y, Ishihara J, Mori T. Comparative lipoquinone analysis of influent sewage and activated sludge by high-performance liquid chromatography and photodiode array detection. J Gen Appl Microbiol 1996; 42:457–469 [CrossRef]
    [Google Scholar]
  23. Fautz E, Reichenbach H. A simple test for flexirubin-type pigments. FEMS Microbiol Lett 1980; 8:87–91 [CrossRef]
    [Google Scholar]
  24. Ngo HTT, Trinh H, Yan Z-F, Moya G, Kook M et al. Niabella hibiscisoli sp. nov., isolated from soil of a Rose of Sharon garden. Int J Syst Evol Microbiol 2017; 67:784–788 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004351
Loading
/content/journal/ijsem/10.1099/ijsem.0.004351
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error