1887

Abstract

A polyphasic study was undertaken to establish the position of a strain, isolate PRKS01-65, recovered from sand dune soil collected at Parangkusumo, Yogyakarta Province, Java, Indonesia. A combination of chemotaxonomic, cultural and morphological properties confirmed its position in the genus of . Comparative 16S rRNA gene sequence analyses showed that the isolate was most closely related to C34 (99.9 % similarity) and loosely associated with the type strains of (98.7 % similarity) and (98.9 % similarity). Multilocus sequence analyses based on five conserved housekeeping gene alleles confirmed the close relationship between the isolate and C34, although both strains belonged to a well-supported clade that encompassed the type strains of , , , , and . A comparison of the draft genome sequence generated for the isolate with corresponding whole genome sequences of its closest phylogenomic neighbours showed that it formed a well-separated lineage with C34. These strains can also be distinguished using a combination of phenotypic properties and by average nucleotide identity and digital DNA–DNA hybridization similarities of 94.3 and 56 %, values consistent with their classification in different species. Based on this wealth of data it is proposed that isolate PRKS01-65 (=NCIMB 15211=CCMM B1302=ICEBB-03) be classified as sp. nov. The genome of the isolate contains several biosynthetic gene clusters with the potential to produce new natural products.

Funding
This study was supported by the:
  • Indonesia Endowment Fund for Education (LPDP) (Award PhD scholarship)
    • Principle Award Recipient: Ali Budhi Kusuma
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2020-08-21
2021-08-02
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