1887

Abstract

Three strains of a Gram-stain-positive, catalase-negative, facultative anaerobic, and coccoid species were isolated from German bulk tank milk. Phylogenetic analyses based on the 16S rRNA gene sequences indicated that the three strains (WS4937, WS4759 and WS5303) constitute an independent phylogenetic lineage within the family with CCUG 36813 (93.7–94.1 %) and M1831/95/2 (93.5 %) as most closely related type species. The unclassified strains demonstrated variable growth with 6.5 % (w/v) NaCl and tolerated pH 6.5–9.5. Growth was observed from 12 to 39 °C. Their cell-wall peptidoglycan belongs to the A1 type (-Lys-direct) consisting of alanine, glutamic acid and lysine. The predominant fatty acids were C ω9, C and C ω9 and in the polar lipids profile three glycolipids, a phospholipid, phosphatidylglycerol, phosphoglycolipid and diphosphatidylglycerol were found. The G+C content of strain WS4937 was 37.4 mol% with a genome size of ~3.0 Mb. Based on phylogenetic, phylogenomic and biochemical characterizations, the isolates can be demarcated from all other genera of the family and, therefore, the novel genus gen. nov. is proposed. The type species of the novel genus is gen. nov., sp. nov. WS4937 (=DSM 109652=LMG 31441).

Funding
This study was supported by the:
  • Annemarie Siebert , Bundesanstalt für Landwirtschaft und Ernährung , (Award 281A105616)
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2020-08-03
2020-09-20
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