@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004342, author = "Liou, Jong-Shian and Huang, Chien-Hsun and Ikeyama, Nao and Lee, Ai-Yun and Chen, I-Ching and Blom, Jochen and Chen, Chih-Chieh and Chen, Chia-Hsuan and Lin, Yu-Chun and Hsieh, Sung-Yuan and Huang, Lina and Ohkuma, Moriya and Watanabe, Koichi and Sakamoto, Mitsuo", title = "Prevotella hominis sp. nov., isolated from human faeces", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "8", pages = "4767-4773", doi = "https://doi.org/10.1099/ijsem.0.004342", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004342", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "gut microbiota", keywords = "Prevotella", keywords = "culturomics", abstract = "A strictly anaerobic predominant bacterium, designated as strain gm001T, was isolated from a freshly voided faecal sample collected from a healthy Taiwanese adult. Cells were Gram-stain-negative rods, non-motile and non-spore-forming. Strain gm001T was identified as a member of the genus Prevotella , and a comparison of 16S rRNA and hsp60 gene sequences revealed sequence similarities of 98.5 and 93.3 %, respectively, demonstrating that it was most closely related to the type strain of Prevotella copri . Phylogenomic tree analysis indicated that the gm001T cluster is an independent lineage of P. copri DSM 18205T. The average nucleotide identity, digital DNA‒DNA hybridization and average amino acid identity values between strain gm001T and P. copri DSM 18205T were 80.9, 28.6 and 83.8 %, respectively, which were clearly lower than the species delineation thresholds. The species-specific genes of this novel species were also identified on the basis of pan-genomic analysis. The predominant menaquinones were MK-11 and MK-12, and the predominant fatty acids were anteiso-C15 : 0, C15 : 0 and iso-C15 : 0. Acetate and succinate were produced from glucose as metabolic end products. Taken together, the results indicate that strain gm001T represents a novel species of the genus Prevotella , for which the name Prevotella hominis sp. nov. is proposed. The type strain is gm001T (=BCRC 81118T=JCM 33280T).", }