1887

Abstract

An anaerobic bacterial strain, named TLL-A4, was isolated from fecal pellets of conventionally raised C57BL/6J mice. Analysis of the 16S rRNA gene indicated that the strain belongs to the phylum Bacteroidetes and, more specifically, to the recently proposed (also known as S24-7 clade or Homeothermaceae). Strain TLL-A4’s 16S rRNA gene shared 92.8 % sequence identity with the type strain of the only published species of the genus , DSM 28989. Genome-sequencing of TLL-A4 was performed to compare average amino acid identity (AAI) value and percentage of conserved proteins (POCP) between both strains. The AAI analysis revealed that strain TLL-A4 had high identity (69.8 %) with DSM 28989, while the POCP was 56 %. These values indicate that strain TLL-A4 could be considered a member of the genus but not belonging to the species . Quinone analysis indicated MK10 (63 %) and MK11 (32 %) as major quinones in the membrane, while MK9 was only present as a minor component (5 %). The main cellular fatty acid was anteiso-C (42.8 %); summed feature 11 (17.5 %), C iso (13.4 %), C ω9 (5.6 %), C 3-OH (4.5 %) and C (4.2 %) were detected in minor amounts. Analysis of enzyme activities using the API 32A and API 20A kits indicated major differences between strain TLL-A4 and DSM 28989. Based on genotypic, phylogenetic and phenotypic differences, strain TLL-A4 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TLL-A4 (=DSM 108194=KCTC 15770).

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2020-07-20
2020-08-11
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