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Volume 70, Issue 8

sp. nov., a halotolerant actinobacterium isolated from saline lake sediment Free

Abstract

A novel Gram-stain-positive bacterium, designated CFH 91151, was isolated from sediment collected from a saline lake in Yuncheng, Shanxi Province, PR China. Cells of strain CFH 91151 were rod-or v-shaped, aerobic, non-motile, non-spore-forming and halotolerant. Results of 16S rRNA gene sequence analysis revealed that strain CFH 91151 was closely related to MX5 and H17 (98.7 and 98.4% sequence similarity, respectively). The strain grew at 4–45 °C, pH 5.0–9.0 and with 0–14.0 % (w/v) NaCl. Cells were positive for catalase, nitrate was not used and HS was not produced. Major cellular fatty acids were anteiso-C (62.76 %), anteiso-C (12.09 %) and iso-C (9.46 %). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, two unidentified phospholipids and three unidentified glycolipids. The menaquinone was MK-9 (H4). The genome size was 4.10 Mbp with a G+C content of 72.4 mol%. The average amino acid identity (ANI) and DNA–DNA hybridization (DDH) values between CFH 91151 and the other species of the genus were found to be low (ANIm <87.19 %, ANIb <84.38 % and DDH <29.30 %). Based on physiological properties, chemotaxonomic characteristics and low ANI and DDH results, strain CFH 91151 is considered to represent a novel species, for which the name sp. nov. is proposed. The type strain is CFH 91151 (=DSM 105976=KCTC 49061).

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  • Accepted:
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Keyword(s): Isoptericola halalbus sp. nov. , polyphasic taxonomy and saline lake
Funding
This study was supported by the:
  • Doctor Scientific Research Fund of Xinxiang Medical University (Award XYBSKYZZ201625)
    • Principle Award Recipient: Hong Ming
  • Henan Province University youth researcher support project (Award 2017GGJS106)
    • Principle Award Recipient: Hong Ming
  • Innovation Scientists and Technicians Troop Construction Projects of Henan Province (Award CXTD2016043)
    • Principle Award Recipient: Guo-Xing Nie
  • Natural Science Foundation of China (Award 3150004)
    • Principle Award Recipient: Hong Ming
© 2020 The Authors
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2020-07-20
2024-04-18
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Isoptericola halalbus sp. nov., a halotolerant actinobacterium isolated from saline lake sediment
Int J Syst Evol Microbiol 70, 4661 (2020); https://doi.org/10.1099/ijsem.0.004329
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