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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 70, Issue 8

Genome-based reclassification of DSM 21345 as subsp. subsp. nov. and description of subsp. subsp. nov. Free

Abstract

Bacteria of the genus are relevant to humans, animals and plants. The species and are Gram-stain-positive and endospore-forming bacilli isolated from a blood culture of a leukemia patient and from soil of a ginseng field, respectively. Comparative analyses of their 16S rRNA genes revealed that the two species could be synonyms (99.3% sequence identity). In the present study we performed different genomic analyses in order to evaluate the phylogenetic relationship of these micro-organisms. DSM 16942 and DSM 21345 presented a difference in their G+C content lower than 1 mol%, overall genome relatedness index values higher than the species circumscription thresholds (average nucleotide identity, 95.57 %; genome-wide ANI, =96.51 %; and orthologous ANI, 96.25 %), and a monophyletic grouping pattern in the phylogenies of the 16S rRNA gene and the proteome core. Considering that these strains present differential biochemical capabilities and that their computed digital DNA–DNA hybridization value is lower than the cut-off for bacterial subspecies circumscription, we suggest that each of them form different subspecies of , subsp. subsp. nov. (type strain DSM 21345) and subsp. subsp. nov. (type strain DSM 16942).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA phylogeny , genome metrics , Genomic metrics , OGRI , overall genome relatedness index , Proteoma-core phylogeny , reclassification and taxonomy
Funding
This study was supported by the:
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico
    • Principle Award Recipient: Fabiana Tonial
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
    • Principle Award Recipient: Fabiana Tonial
© 2020 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.004328
2020-07-21
2024-04-23
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Genome-based reclassification of Paenibacillus panacisoli DSM 21345T as Paenibacillus massiliensis subsp. panacisoli subsp. nov. and description of Paenibacillus massiliensis subsp. massiliensis subsp. nov.
Int J Syst Evol Microbiol 70, 4838 (2020); https://doi.org/10.1099/ijsem.0.004328
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