1887

Abstract

Six strains were isolated from healthy black bears () in the Great Smoky Mountains National Park, Tennessee, USA. Phylogenetic analysis based on complete genome, 16S rRNA, , , and genes, and MALDI-TOF-MS main spectral profiles revealed that the strains belonged to one species and showed the closest relatedness to members of the ‘ group’ (SIG), which include , and . The strains were positive in SIG-specific and negative in individual species-specific PCR assays for the gene. The strains can be differentiated from the other SIG species by the absence of sucrose fermentation, from DSM 20373, CCUG 49543 and DSM 105366 by the absence of methyl β--glucopyranoside fermentation and from DSM 20771 by fermentation of trehalose. DNA relatedness of the type strain MI 10-1553 with the type strains of , , and was ≤48.2 % by digital DNA–DNA hybridization and ≤92.3 % by average nucleotide identity calculations. Iso-, anteiso-C and anteiso-C were the most common fatty acids. Polar lipids consisted of phosphadidylglycerols, phospholipids, glycolipid, diphosphatidylglycerol and aminophospholipid. Cell-wall peptidoglycan was of type A3α -Lys-Gly (Ser; similar to A11.2 and A11.3). The respiratory quinone belonged to menaquinone 7 (MK-7). The G+C content of MI 10-1553 was 39.3 mol%. The isolated strains represent a novel species of the genus , for which we propose the name sp. nov. The type strain is MI 10-1553 (=ATCC TSD-55=CCOS 1900).

Funding
This study was supported by the:
  • All Taxa Biodiversity Inventory Grant from Discover Life in America (Award DLIA2010-07)
    • Principle Award Recipient: Stephen A Kania
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2020-07-16
2021-08-02
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