@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.004321, author = "Seo, Ye Lin and Khan, Shehzad Abid and Kim, Hyung Min and Chun, Byung Hee and Han, Dong Min and Jeon, Che Ok", title = "Mucilaginibacter agri sp. nov. and Mucilaginibacter humi sp. nov., isolated from soil", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2020", volume = "70", number = "8", pages = "4616-4622", doi = "https://doi.org/10.1099/ijsem.0.004321", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.004321", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Mucilaginobacter humi", keywords = "bacteroidetes", keywords = "new taxa", keywords = "Mucilaginibacter agri", keywords = "soil", abstract = "Two Gram-stain-negative, facultative anaerobic and non-motile bacteria, strains R11T and S1162T, were isolated from soil in the Republic of Korea. Both strains were catalase- and oxidase-positive and contained menaquinone-7 as the major isoprenoid quinone. Strain R11T contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), iso-C15:0, C16:0 and iso-C17:0 3-OH as major fatty acids and phosphatidylethanolamine, an unidentified aminophospholipid and an unidentified aminolipid as major polar lipids; while strain S1162T contained summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), iso-C15:0, iso-C17:0 3-OH, C16:0 and summed feature 9 (10-methyl C16:0 and/or iso-C17:1 ω9c) as major fatty acids and phosphatidylethanolamine and an unidentified aminophospholipid as major polar lipids. The DNA G+C contents of strains R11T and S1162T calculated from their whole genomes were 42.7 and 42.9 mol%, respectively. Results of phylogenetic analysis based on 16S rRNA gene sequences showed that strain R11T formed a phylogenetic lineage with Mucilaginibacter jinjuensis YC7004T and strain S1162T formed a distinct phyletic lineage from closely related type strains within the genus Mucilaginibacter . Strains R11T and S1162T were most closely related to M. jinjuensis YC7004T and Mucilaginibacter panaciglaebae BXN5-31T with 97.78 and 97.23% 16S rRNA gene sequence similarities, respectively. On the basis of phenotypic, chemotaxonomic and molecular analysis, strains R11T and S1162T represent two novel species of the genus Mucilaginibacter , for which the names Mucilaginibacter agri sp. nov. and Mucilaginibacter humi sp. nov. are proposed, respectively. The type strains of M. agri and M. humi are R11T (=KACC 21228T=JCM 33472T) and S1162T (=KACC 21669T=JCM 33916T), respectively.", }