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Volume 70, Issue 8

gen. nov., sp. nov., a member of the family isolated from a deep-sea seamount Free

Abstract

The Gram-stain-negative, orange-pigmented, non-spore-forming, non-motile, strictly aerobic, rod-shaped bacterial strain, designated TP-CH-4, was isolated from a seamount near the Yap Trench in the tropical western Pacific. The optimal growth conditions were determined to be at pH 7–8, 25–30 °C and in the presence of 2 % (w/v) NaCl. The major respiratory quinone was MK-6. The polar lipid profile contained phosphatidylethanolamine, two unidentified aminolipids, two unidentified phospholipids and three unidentified polar lipids. The predominant cellular fatty acids were iso-C and summed feature 1 (composed of C3-OH and/or iso-CH). Phylogenetic analysis of 16S rRNA gene sequences revealed that strain TP-CH-4 was a member of the family and formed a distinct lineage. Strain TP-CH-4 displayed highest sequence similarities to KMM 3531 (95.1 %) and A11 (93.9 %). Genome sequencing revealed the strain TP-CH-4 has a genome size of 4.5 Mbp and a G+C content of 44.5 mol%. Collectively, based on phenotypic, chemotaxonomic, phylogenetic and genomic evidence, strain TP-CH-4 represents a novel species of a novel genus of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is TP-CH-4 (=CGMCC 1.17120=KCTC 72434).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Flavobacteriaceae and seamount
Funding
This study was supported by the:
  • the Key Research Program of Frontier Sciences, Chinese Academy of Sciences (Award QYZDB-SSW-DQC023)
    • Principle Award Recipient: Feng Liu
  • the Science & Technology Basic Resources Investigation Program of China (Award 2017FY100804)
    • Principle Award Recipient: De-Chao Zhang
  • the Senior User Project of RV KEXUE (Award KEXUE2019G09)
© 2020 The Authors
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2020-07-07
2024-04-18
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Pelagihabitans pacificus gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from a deep-sea seamount
Int J Syst Evol Microbiol 70, 4569 (2020); https://doi.org/10.1099/ijsem.0.004315
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